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G-Protein Coupled Receptors >> Glutamate Receptors >> Antibodies to mGluRs

Anti-mGluR2 (extracellular)

Metabotropic Glutamate receptor 2, GRM2, mGlu2
  • Antigen included
    Antigen included
  • Shipped at room temp.
    Shipped at room temp.
  • QC tested
    QC tested
  • Live cell Imaging
    Live cell Imaging
  • Cat #: AGC-011
  • Size: 50 µl, 0.2 ml
  • Source: Rabbit
  • Type:  Polyclonal
Application: IC, IH, LCI, WB
 
Reactivity: H, M, R
May also work in: IFC, IP
 
Application key: CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry,
IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation,
LCI- Live cell imaging, N- Neutralization, WB- Western blot     
Species reactivity key: H- Human, M- Mouse, R- Rat
Control antigen included Lyophilized powder
General information
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat mGluR2 receptor. The epitope is specific for mGluR2 and will not cross-react with the closely related mGluR3 channel. Anti-mGluR2 (extracellular) antibody (#AGC-011) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize mGluR2 from human, rat and mouse samples.

Related products for control experiments:
Control peptide antigen (supplied with the antibody free of charge).

If you know of a relevant citation for this product please let us know.
Applications
Specifications
Scientific Background
 
Western blot
Anti-mGluR2_(extracellular) - Western blot analysis of rat cerebellum (lanes 1 and 3) and cortex (lanes 2 and 4) membranes:
Western blot analysis of rat cerebellum (lanes 1 and 3) and cortex (lanes 2 and 4) membranes:
1, 2. Anti-mGluR2 (extracellular) antibody (#AGC-011), (1:400).
3, 4. Anti-mGluR2 (extracellular) antibody, preincubated with the control peptide antigen.
Anti-mGluR2_(extracellular) - Western blot analysis of mouse brain membranes:
Western blot analysis of mouse brain membranes:
1. Anti-mGluR2 (extracellular) antibody (#AGC-011), (1:200).
2. Anti-mGluR2 antibody, preincubated with the control peptide antigen.
Live cell imaging / Immunocytochemistry
Anti-mGluR2_(extracellular) - Expression of mGluR2 in rat PC12 cells
Expression of mGluR2 in rat PC12 cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-mGluR2 (extracellular) antibody (#AGC-011), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
Immunohistochemistry
Anti-mGluR2_(extracellular) - Expression of mGluR2 in rat brain
Expression of mGluR2 in rat brain
Immunohistochemical staining of perfusion-fixed brain frozen sections using Anti-mGluR2 (extracellular) antibody (#AGC-011). A. mGluR2 (green) is visualized in the corpus callosum (CC) and hippocampal stratum oriens (OR). B. Glial fibrillary acidic protein (GFAP) (red), a marker of astrocytes. C. Merge of the two images demonstrates expression of mGluR2 in astrocytes. DAPI is used as the nuclear counterstain (blue).
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For research purposes only, not for human use
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