- Cat #:
ALR-001
- Size:
50 µl, 0.2 ml
- Source:
Rabbit
- Type:
Polyclonal
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Application:
IFC, WB
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Reactivity:
H
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Application key: CBE- Cell-based ELISA, FC-
Flow cytometry, IC- Immunocytochemistry,
IE- Indirect ELISA, IFC- Indirect flow
cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation,
LCI- Live cell imaging,
N- Neutralization, WB- Western blot
Species reactivity key: H- Human, M- Mouse,
R- Rat
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Control antigen included
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Lyophilized powder |
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General information
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Leukotrienes (LTs) are lipid mediators prominently exerting proinflammatory responses. They are divided in two classes: cysteinyl LTs (cysLTs) which have a thioester linkage and LTB41. LTB4, a chemoattractant plays a prominent role in the recruitment and activation of leukocytes2.
LTB4 acts through two receptors which belong to the G-protein coupled receptor superfamily: BLT1 and BLT2. BLT1 displays high affinity for LTB4 and is highly expressed in leukocytes including granulocytes, macrophages, mast cells, dendritic cells and effector T cells3 and is expressed to much lower levels in spleen, thymus, heart, skeletal muscle brain and liver2-4. BLT2 on the other hand is a low affinity receptor which does not display selectivity towards LTB45. Its expression is much more ubiquitous than that of BLT1. It is expressed in most human tissues, with the highest expression being in spleen, liver, ovary, and leukocytes2,5-8. The two receptors could couple and activate different G-proteins, depending on the cell type and the cellular events activating the receptors2. However, activation of BLT1 ususally correlates with increasing intracellular concentrations of Ca2+.
The LTB4/BLT1 system is involved in many allergic reactions as well as asthma induced as an allergic response, where it plays a significant role in recruiting neutrophils and effector T cells into lungs, as part of an inflammatory response induced by allergens1.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of human BLT1. Anti-Human BLT1 (extracellular) antibody (#ALR-001) can be used for Western blot analysis and indirect flow cytometry applications. It has been designed to recognize BLT1 from human samples only.
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| Western blot |
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| Western blot analysis of acute T-cell leukemia Jurkat (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute monocytic leukemia THP-1 (lanes 3 and 6) human cell lysates: |
1-3. Anti-Human BLT1 (extracellular) antibody (#ALR-001), (1:200).
4-6. Anti-Human BLT1 (extracellular) antibody, preincubated with the control peptide antigen. |
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| Indirect flow cytometry |
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| Indirect flow cytometry analysis in live intact human T cell leukemia (Jurkat) cell line: |
___ Control cells + goat-anti-rabbit-Cy5.
___ Cells + Anti-Human BLT1 (extracellular) antibody (#ALR-001), (1:25) + goat-anti-rabbit-Cy5. |
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| Indirect flow cytometry analysis in live intact human acute monocytic leukemia (THP-1) cell line: |
___ Control cells + goat-anti-rabbit-Cy5.
___ Cells + Anti-Human BLT1 (extracellular) antibody (#ALR-001), (1:25) + goat-anti-rabbit-Cy5. |
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| Immunogen |
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| Peptide CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human BLT1 (Accession Q15722). 2nd extracellular loop. |
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| Homology |
Monkey - identical. |
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| Specificity |
Not recommended for use with rat and mouse samples. |
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| Purity |
Affinity purified on immobilized antigen. |
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| Standard quality control of each lot |
Western blot analysis. |
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| References |
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| 1. Riccioni, G. et al. (2008) J. Leukoc. Biol. 84, 1374. |
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| 2. Tager, A.M. and Luster A.D. (2003) Prostaglandins Leukot. Essent. Fatty Acids 69, 123. |
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| 3. Yokomizo, T. et al. (1997) Nature 387, 620. |
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| 4. Owman, C. et al. (1996) Genomics 37, 187. |
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| 5. Yokomizo, T. et al. (2000) J. Exp. Med. 192, 421. |
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| 6. Tryselius, Y. et al. (2000) Biochem. Biophys. Res. Commun. 274, 377. |
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| 7. Kamohara, M. et al. (2000) J. Biol. Chem. 275, 27000. |
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| 8. Wang, S. et al. (2000) J. Biol. Chem. 275, 39482. |
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