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Ion Channels >> Cell Signaling Tools >> Mitochondrial ATPase >> Inhibitors

Oligomycin

A Mitochondrial ATP Synthase Inhibitor
  • Bioassay tested
    Bioassay tested
  • Shipped at room temp.
    Shipped at room temp.
  • Cat #: O-500
  • Size: 5 mg, 5 x 5 mg, 10 x 1 mg
  • Source: Natural
  • MW: 789.3 Da.
General information
Oligomycin is a natural antibiotic isolated from Streptomyces diastatochromogenes which inhibits mitochondrial H+-ATP synthase. It is primarily found to act as an inhibitor of mitochondrial respiration and swelling. This antibiotic is widely used as an inhibitor of oxidative phosphorylation.1-2

Oligomycin inhibits the H+- ATP-synthase by binding to the Oligomycin sensitivity-conferring protein (OSCP) at the F(o) subunits 6 and 9  which are found in the stalk of the F1F0-ATPase complex. This binding blocks the proton conductance and inhibits the synthesis of mitochondrial ATP.3-4 Because of its activity, it can also be used to reduce the number of parameters (such as ER Ca2+ release, exocytotoxicity and apopotosis) which are affected by mitochondrial depolarization.5

Oligomycin, at high concentrations may also inhibit the plasma membrane Na+-K+-ATPase. Interaction of Oligomycin with the Na+ occlusion site on the extracellular side of Na/K-ATPase, delays Na+ release to the extracellular side without inducing a conformational change. Although Oligomycin stimulated Na+ binding to Na+/K+-ATPase, it inhibited  Na+/Na+ exchange, and did not affect either Na+-dependent AD/-ATP exchange or K+-dependent phosphatase activity.6-16

These inhibitory properties have been attributed to its ability to occlude the transition of E1~P (high energy intermediate) to E2-P (low energy intermediate) by oligomycin binding to E1~P.17

Alomone Labs is pleased to offer Oligomycin (#O-500), isolated and purified from Streptomyces diastatochromogenes.
Our Bioassay
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References
 
Our bioassay
Oligomycin_ - Alomone Labs Oligomycin inhibits ATP-dependent adipogenesis in 3T3-L1 cells.
Alomone Labs Oligomycin inhibits ATP-dependent adipogenesis in 3T3-L1 cells.
Cells were induced to undergo adipocyte differentiation (A-D) for 5 days. Different concentrations of Oligomycin (#O-500) were then added (B, 1 µg/ml; C, 2 µg/ml and D, 10 µg/ml). After 10 days, the cells were stained with Oil Red O and visualized under light microscopy.
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