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- Peptide (C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 muscarinic receptor (Accession P11229). Intracellular, C-terminus.
- Western blot analysis of rat brain lysate:1. Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:200).
2. Anti-CHRM1 (443-458) Antibody, preincubated with the negative control antigen.
- Expression of Muscarinic acetylcholine receptor M1 in mouse striatumImmunohistochemical staining of mouse striatum using Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:100). M1 mAChR staining (red) appears in the matrix (M), not in the patches (P). Blood vessels in the matrix (arrow) are also stained. DAPI is used as the counterstain (blue).
- 1. Felder, C.C. et al. (2000) J. Med. Chem. 43, 4333.
- 2. Forsythe, S.M. et al. (2002) Am. J. Respir. Cell. Mol. Biol. 26, 298.
- 3. Ferreira, A.R. et al. (2003) Pharmacol. Biochem. Behav. 74, 411.
- 4. Van der Zee, E.A. et al. (1999) Prog. Neurol. 58, 409.
- 5. Levey, A.I. et al. (1991) J. Neurosci. 11, 3218.
- 6. Wang, J. et al. (2000) Am. J. Physiol. Gastrointest. Liver Physiol. 279, G1059.
- 7. Budd, S.C. et al. (2003) J. Biol. Chem. 278, 19565.
The action of the neurotransmitter acetylcholine (ACh) is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named M1-M5.1-2
The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exert both excitatory and inhibitory control over central and peripheral tissues.1-2
Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.1 They also participate in learning and memory processing.3-4 The M1 receptors are the most abundant muscarinic subtype in the cortex and striatum. M1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of ACh from the nerves.5-6
The M1, M3 and M5 receptors, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the M1, M3 and M5 receptors is an essential element for the anti-apoptotic response of those receptors.7
Species reactivity key:
Anti-CHRM1 (443-458) Antibody (#AMR-010) is a highly specific antibody directed against an epitope of the human M1 muscarinic receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize M1 receptor from mouse, rat, and human samples.