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G-Protein Coupled Receptors >> Prostanoid Receptors >> Antibodies

Anti-Prostanoid DP2 Receptor (extracellular)

Prostaglandin D2 Receptor 2, CRTH2, GPR44, CD294, PTGDR2
  • Antigen included
    Antigen included
  • Shipped at room temp.
    Shipped at room temp.
  • QC tested
    QC tested
  • Cat #: APR-062
  • Size: 25 µl, 50 µl, 0.2 ml
  • Source: Rabbit
  • Type:  Polyclonal
Application: IFC, IH, WB
 
Reactivity: M, R
May also work in: IC, IP, LCI
 
Application key: CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry,
IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation,
LCI- Live cell imaging, N- Neutralization, WB- Western blot     
Species reactivity key: H- Human, M- Mouse, R- Rat
Control antigen included Lyophilized powder
General information
Anti-Prostanoid DP2 Receptor (extracellular) (#APR-062) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize DP2 Receptor from mouse and rat samples. It is unlikely to recognize the receptor in human samples.
 
Related products for control experiments:
Control peptide antigen (supplied with the antibody free of charge).

If you know of a relevant citation for this product please let us know.
Applications
Specifications
Scientific Background
 
Western blot
Anti-Prostanoid_DP2_Receptor_(extracellular) - Western blot analysis of rat brain lysate:
Western blot analysis of rat brain lysate:
1. Anti-Prostanoid DP2 Receptor (extracellular) antibody (#APR-062), (1:200).
2. Anti-Prostanoid DP2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
Anti-Prostanoid_DP2_Receptor_(extracellular) - Western blot analysis of mouse brain lysate:
Western blot analysis of mouse brain lysate:
1. Anti-Prostanoid DP2 Receptor (extracellular) antibody (#APR-062), (1:400).
2. Anti-Prostanoid DP2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
Immunohistochemistry
Anti-Prostanoid_DP2_Receptor_(extracellular) - Expression of DP2 Receptor in rat medial septum and mouse cerebellum
Expression of DP2 Receptor in rat medial septum and mouse cerebellum
Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-Prostanoid DP2 Receptor (extracellular) antibody (#APR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. DP2 Receptor staining (green) in rat brain sections is detected neuron-like profiles (arrows) in the medial septum/diagonal band. B. DP2 Receptor staining (green) in mouse brain is observed in purkinje cells (horizontal arrow) and of a subset of neurons in the granule layer (GL, vertical arrow). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
Anti-Prostanoid_DP2_Receptor_(extracellular) - Indirect flow cytometry of live intact mouse P815 mast cells:
Indirect flow cytometry of live intact mouse P815 mast cells:
___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Prostanoid DP2 Receptor (extracellular) antibody (#APR-062), (5 µg) + goat-anti-rabbit-FITC.
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For research purposes only, not for human use
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