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Ion Channels >> Intracellular Ion Channels >> Ryanodine Receptor Channels >> Antibodies

Anti-Ryanodine Receptor 2

Cardiac Ryanodine Receptor, RyR2
  • Antigen included
    Antigen included
  • Shipped at room temp.
    Shipped at room temp.
  • QC tested
    QC tested
  • Cat #: ARR-002
  • Size: 25 µl, 50 µl, 0.2 ml
  • Source: Rabbit
  • Type:  Polyclonal
Application: IH, WB
Reactivity: M, R
Application key: CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry,
IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation,
LCI- Live cell imaging, N- Neutralization, WB- Western blot     
Species reactivity key: H- Human, M- Mouse, R- Rat
Control antigen included Lyophilized powder
General information
It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2  The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3

The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.
RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain. 1-4
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the cytoplasmic region of the human Ryanodine receptor 2 (RyR2). The Anti-Ryanodine Receptor 2 antibody (#ARR-002) can be used in western blot analysis and Immunohistochemical applications. It has been designed to recognize RyR2 from human, rat and mouse samples.
Product Citation
Western blot
Anti-Ryanodine_Receptor_2 - Western blot analysis of rat heart membranes:
Western blot analysis of rat heart membranes:
1. Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:200).
2. Anti-Ryanodine Receptor 2 antibody, preincubated with the control peptide antigen.
Anti-Ryanodine_Receptor_2 - Expression of Ryanodine Receptor 2  in rat cardiac muscle
Expression of Ryanodine Receptor 2  in rat cardiac muscle
Immunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
Anti-Ryanodine_Receptor_2 - Expression of Ryanodine Receptor 2 in mouse cerebellum
Expression of Ryanodine Receptor 2 in mouse cerebellum
 Immunohistochemical staining of mouse cerebellum frozen sections with Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:100), (green fluorescence). A. The highest expression of Ryanodine Receptor 2 is in the molecular layer (Asterisk) but there is also some expression in the soma of Purkinje cells (arrows). B. In the same section, there is staining for parvalbumin (red), a marker for Purkinje cells. C. Merged image of panels A and B demonstrates that Ryanodine Receptor 2 is localized both in the area surrounding the dendritic tree and in the soma of Purkinje cells. DAPI is used as the counterstain (blue).
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For research purposes only, not for human use
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