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Ion Channels >> Intracellular Ion Channels >> Ryanodine Receptor Channels >> Antibodies

Anti-Ryanodine Receptor 2

Cardiac Ryanodine Receptor, RyR2
  • Antigen included
    Antigen included
  • Shipped at room temp.
    Shipped at room temp.
  • QC tested
    QC tested
  • Cat #: ARR-002
  • Size: 25 µl, 50 µl, 0.2 ml
  • Source: Rabbit
  • Type:  Polyclonal
Application: IH, WB
 
Reactivity: M, R
Application key: CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry,
IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation,
LCI- Live cell imaging, N- Neutralization, WB- Western blot     
Species reactivity key: H- Human, M- Mouse, R- Rat
Control antigen included Lyophilized powder
General information
It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2  The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3

The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.
 
RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain. 1-4
 
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the cytoplasmic region of the human Ryanodine receptor 2 (RyR2). The Anti-Ryanodine Receptor 2 antibody (#ARR-002) can be used in western blot analysis and Immunohistochemical applications. It has been designed to recognize RyR2 from human, rat and mouse samples.
Applications
Specifications
References
Product Citation
 
Western blot
Anti-Ryanodine_Receptor_2 - Western blot analysis of rat heart membranes:
Western blot analysis of rat heart membranes:
1. Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:200).
2. Anti-Ryanodine Receptor 2 antibody, preincubated with the control peptide antigen.
Immunohistochemistry
Anti-Ryanodine_Receptor_2 - Expression of Ryanodine Receptor 2  in rat cardiac muscle
Expression of Ryanodine Receptor 2  in rat cardiac muscle
Immunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
Anti-Ryanodine_Receptor_2 - Expression of Ryanodine Receptor 2 in mouse cerebellum
Expression of Ryanodine Receptor 2 in mouse cerebellum
 Immunohistochemical staining of mouse cerebellum frozen sections with Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:100), (green fluorescence). A. The highest expression of Ryanodine Receptor 2 is in the molecular layer (Asterisk) but there is also some expression in the soma of Purkinje cells (arrows). B. In the same section, there is staining for parvalbumin (red), a marker for Purkinje cells. C. Merged image of panels A and B demonstrates that Ryanodine Receptor 2 is localized both in the area surrounding the dendritic tree and in the soma of Purkinje cells. DAPI is used as the counterstain (blue).
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For research purposes only, not for human use
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