Anti-Kv7.3 (KCNQ3)


Product#:APC-051

Sizes:
50 ml
0.2 ml


The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5.  Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels.  KCNQ family members can form either homomultimeric or heteromultimeric channels with different functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor.  
Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as benign familial neonatal convulsions (BNFC).

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of rat Kv7.3. The Anti-Kv7.3 (KCNQ3) antibody (#APC-051) can be used for western blot and immunohistochemical applications. It has been designed to recognize Kv7.3 from rat, human and mouse samples.

Host:

Rabbit.

Type:
Polyclonal.

Epitope:
Peptide AEGEKKEDNRYSDLKTIIC, corresponding to amino acid residues 668-686 of rat Kv7.3 (accession number O88944).
Putative epitope location:
Intracellular, C-terminal.
Homology:

Mouse - identical; human - 18/19 amino acid residues identical.

Specificity:
The antibody is specific for KCNQ3, it does not cross react with KCNQ2 or other QKT proteins.

Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
 
Western blot analysis of rat brain membranes:  
1. Anti-Kv7.3 (KCNQ3) antibody (#APC-051), (1:200).   
2. Anti-Kv7.3 (KCNQ3) antibody, preincubated with the control peptide antigen.
Immunohistochemistry:
Expression of Kv7.3 in rat cerebellum
Immunohistochemical staining of rat cerebellum using Anti-Kv7.3 (KCNQ3) antibody (#APC-051), (green). Kv7.3 is localized to the Purkinje cell layer (arrows). Mouse anti-neurofilament 200 (red) localizes Purkinje neurons (arrowheads).


References:
1. Kullmann, D.M. (2002) J. Mol. Brain 125, 1117.  
2. Robbins, J. (2001) Pharmacol. Ther. 90, 1.  
3. Wang, H.S. et al. (1998) Science 282, 1890.  
For research purposes only, not for human use.