Anti-Kir6.2

A Polyclonal Antibody to Kir6.2 (KATP) Inward Rectifier K+ Channel


Product#:APC-020

Sizes:
50 ml
0.2 ml


Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of  
voltage-independent K+ channels largely involved in stabilization of the membrane resting  
potential and in K+ transport across membranes. Kir channels can be modulated by a variety of  
intracellular agents such as protons, GTP-binding proteins and adenine nucleotides.  
The ATP-sensitive channel (KATP) is especially important since it couples cellular 
metabolism (intracellular ATP levels) with cell excitability. KATP channels have been  
described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle.  
The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea  
receptor (SUR) subunit.  
The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and  
SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes. 
Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been  
linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia  
of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk  
of developing type-2 diabetes.

Host:
Rabbit.
Epitope:
Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2  (accession number P70673).
Putative epitope location:
Intracellular, C-terminal part.
Homology:
Mouse - identical; human- 12/14 residues identical.
Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
.
Western blotting of rat pancreas membranes: 
1. Anti-Kir6.2 antibody (1:200).   
2. Anti-Kir6.2 antibody, preincubated with the control peptide antigen.
Immunohistochemistry:
 
Staining of rat pancreas with Anti-Kir6.2 antibody (#APC-020). (A) Strong granular staining in a number of cells within the Islets of Langerhans is readily detected (red). (B) The negative control slide shows no staining.

References:
1. Babenko, A.P. et al. (1998) Annu. Rev. Physiol. 60, 667.
2. Huopio, H. et al. (2002) Am. J. Physiol. Endocrinol. Metab. 283, 207.
For research purposes only, not for human use.