Anti-Kv7.2 (KCNQ2)


Product#:APC-050

Sizes:
50 ml
0.2 ml


The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5.  
Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels.  
KCNQ family members can form either homomultimeric or heteromultimeric channels with different  
functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much  
larger channel current than when either protein is expressed alone, probably due to enhanced  
plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are  
believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current  
that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor.  
Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as benign familial neonatal convulsions (BNFC).

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of rat Kv7.2. The Anti-Kv7.2 (KCNQ2) antibody (#APC-050) can be used for western blot analysis. It has been designed to recognize Kv7.2 from rat, human and mouse samples.

Host:

Rabbit.

Type:
Polyclonal.

Epitope:
Peptide (C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2 (Accession number O88943).
Putative epitope location:
Intracellular, C-terminus.
Homology:

Mouse - identical; human - 15/16 amino acid residues identical.

Specificity:  
The antibody is specific for KCNQ2; it does not cross react with KCNQ3 or other QKT proteins. 
There are at least 9 recognized splice variants of rat KCNQ2.  This antibody recognizes all except splice variants B and C. 

Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
 
Western blot analysis of Kv7.2 transfected HEK-293 cells:  
1. Anti-Kv7.2 (KCNQ2) antibody (#APC-050), (1:200).   
2. Anti-Kv7.2 (KCNQ2) antibody, preincubated with the control peptide antigen.

References:
1. Kullmann, D.M. (2002) J. Mol. Brain 125, 1117.  
2. Robbins, J. (2001) Pharmacol. Ther. 90, 1.  
3. Wang, H.S. et al. (1998) Science 282, 1890.  
For research purposes only, not for human use.