Anti-Kv3.3


Product#:APC-102

Sizes:
50 ml
0.2 ml


Kv3.3 is a member of the Shaw type family of voltage-gated K+ channels that includes four members (Kv3.1-3.4). 
Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of  
action potentials and to facilitate repetitive high frequency firing.  
Kv3.3 is mostly localized in the brain, although it has been observed in vascular smooth muscle cells and eye epithelium.  
In the brain its expression pattern largely overlaps that of the Kv3.1 channel suggesting that they may form functional  
heteromers. Indeed, mouse knockouts of both Kv3.1 and Kv3.3, but not either channel alone, display severe motor defects.

Host:

Rabbit.

Type:
Polyclonal.

Epitope:
Peptide KSPITPGSRGRYSRDRAC, corresponding to 
amino acid residues 701-718 of rat Kv3.3 (Accession number Q01956).
Putative epitope location:
Intracellular, C-terminus.
Homology:

Human, mouse - identical.

Specificity:  
Recognizes only Kv3.3-1 splice variant .

Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
Rat brain membrane (1:200).
Western blotting of rat brain membranes:
1. Anti-Kv3.3 antibody (1:200).  
2. Anti-Kv3.3 antibody, preincubated with the control peptide antigen.
Immunohistochemistry:
Expression of Kv3.3 in rat brain
Immunohistochemical staining of rat brain sections using Anti-Kv3.3 antibody (#APC-102). Immunoreactive neurons in the reticular thalamic nucleus detected with diaminobenzidine.  
IH photograph kindly contributed by Jim Deuchars, Leeds, UK.

References:
1. Rudy, B. et al. (2001) Trends Neurosci. 24, 517.  
2. Matsukawa, H. et al. (2003) J Neurosci. 23, 7677.  
For research purposes only, not for human use.