Anti-ASIC1

(Amiloride-sensitive brain sodium channel, BnaC2, ACCN2)


Product#:ASC-014

Sizes:
50 ml
0.2 ml


ASIC1 is a member of a family of Na+ channels that are activated by external protons.  
The family includes another three members ASIC2, ASIC3 and ASIC4.  
The ASICs are in fact part of a larger superfamily termed epithelial Na+ channels (EnaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini.  
There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions.  
The ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium.  
Lately, ASIC1 has been implicated in processes such as learning and memory in the central nervous system. 
We are please to offer our new anti-ASIC1 antibody. The antibody was raised against an epitope in the C-terminus of the rat protein and thus recognizes both ASIC1 isoforms.  
The antibody will also work with human samples.

Host:
Rabbit.
Epitope:
Peptide CQKEAKRSSADKGVALSLDD corresponding to residues 469-488 of rat ASIC1 (accession number P55926).
Putative epitope location:
Intracellular, C-terminus.
Homology:

Human-identical, mouse – 19 out of 20 residues identical.

Antibody Specificity:

Recognizes isoforms ASIC1a and ASIC1b.

Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
Rat brain membranes (1:200).
Western blotting of rat brain membranes: 
1. Anti-ASIC1 antibody (1:200)   
2. Anti-ASIC1 antibody, preincubated with the control peptide antigen
Immunohistochemistry:
Rat brain sections.

Staining of ASIC1 with Anti-ASIC1 antibody (#ASC-014) in rat globus pallidus.  
In picture B, neurons with typically enmeshed dendritic trees stain intensely for ASIC1 (B,red).  
In picture C, double labeling with mouse anti paralbumin (green) reveals strong co-localization of PV with ASIC1.
In A, parvalbumin postive neurons are shown separately.

Control antigen included in price

References:

1.

Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735.  

2.

Wemmie, J. A. et al. (2002) Neuron 34, 463.  

3.

Wemmie, J. A. et al. (2003) J. Neurosci. 23, 5496.
For research purposes only, not for human use.