Anti-Kv1.3 (extracellular)


Product#:APC-101

Sizes:
50 ml
0.2 ml


Kv1.3 belongs to the Shaker family of voltage-dependent K+ channels. 
The channel is widely expressed in the brain, lung and osteoclasts and in several cell  
populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that  
Kv1.3 function has centered a lot of attention. It was found that Kv1.3 is the main channel  
responsible for maintaining the resting potential in quiescent cells and regulating the  
Ca2+ signaling that is indispensable for normal T lymphocyte activation.  
Based on the central role of Kv1.3 in regulating the initiation of an immune response,  
the channel has been recognized as a potential target for immunosuppressant drugs. 
The central role of Kv1.3 in immune system cells created a real need for a specific  
antibody that would be able to work in flow cytometry applications.  

Alomone Labs  is pleased to offer a highly specific antibody directed against an extracellular epitope of human Kv1.3. The Anti-Kv1.3 (extracellular) antibody (#APC-101) can be used for western blot, immunocytochemical and flow cytometry applications. The antibody can also be obtained labeled with fluorescein (FITC), (#APC-101-F).



Host:

Rabbit.

Type:
Polyclonal.

Epitope:
Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human Kv1.3. (accession number P22001).
Putative epitope location:
Extracellular loop between domains S1 and S2.
Homology:
Rat, mouse - 12/14 amino acid residues identical.
Reactivity Confirmed:
Rat, human.
Applications:
Western Blotting:
 
Western blot analysis of rat brain membranes:  
1. Anti-Kv1.3 (extracellular) antibody  (#APC-101), (1:500).  
2. Anti-Kv1.3 (extracellular) antibody, preincubated with the control peptide antigen.
Flow Cytometry:

 

Indirect flow cytometry analysis of live intact human T cell leukemia (Jurkat) cell line:

___ Unstained cells + goat-anti-rabbit-PE.
___ Cells + Anti-Kv1.3 (extracellular) antibody (#APC-101), (1:25) + goat-anti-rabbit-PE.


References:
1. Chandy, K.G.et al. (2001) Toxicon 39, 1269.  
2. Koo, G.C. et al. (1997) J Immunol. 158, 5120.  
For research purposes only, not for human use.