Anti-Kv1.3 (extracellular)-FITC

(Voltage-gated potassium channel KV1.3, Kcna3)


Product#:APC-101-F

Sizes:
50 ml


KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. 
The channel is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that Kv1.3 function has centered a lot of attention. It was found that Kv1.3 is the main channel  
responsible for maintaining the resting potential in quiescent cells and regulating the  
Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2  
Based on the central role of KV1.3 in regulating the initiation of an immune response,  
the channel has been recognized as a potential target for immunosuppressant drugs.1

KV1.3 channels are potently inhibited by several venomous peptide toxins among them
rCharybdotoxin (#RTC-325), rNoxiustoxin (#RTN-340), rKaliotoxin (#RTK-370),
rMargatoxin (#RTM-325), rAgitoxin-1 (#RTA-150), rAgitoxin-2 (#RTA-420),
rHongotoxin-1 (#RTH-400)  and rStichodactyla Toxin (#RTS-400).3-7

The central role of KV1.3 in immune system cells created a real need for a specific  
antibody that would be able to work in flow cytometry applications.  
Alomone has now developed an anti-KV1.3 antibody directed against a specific  
extracellular epitope of the channel.  
The antibody can be obtained in a purified format (#APC-101) or labeled with  
fluorescein (#APC-101F). The antibodies have been tested in flow cytometry,  
immunocytochemistry and western blot applications.



Host:

Rabbit.

Type:
Polyclonal.

Epitope:
Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (accession number P22001).
Putative epitope location:
Extracellular loop between domains S1 and S2.
Homology:

Rat, mouse - 12/14 amino acid residues identical.


Label:
Fluorescein isothiocyanate (FITC).

Reactivity Confirmed:

Rat, human.


Standard quality control of each lot:

Western blot analysis (unlabeled antibody, see datasheet of product (#APC-101) and direct flow cytometry (FITC-labeled antibody).

Applications:
Flow Cytometry:

Direct flow cytometry of live intact Jurkat (human T cell leukemia) cells (0.5-5 mg antibody /1x106cells):
____ Unstained cells.
____ Cells + Anti-Kv1.3 (extracellular)-FITC antibody (#APC-101-F).


References:
1. Chandy, K.G.et al. (2001) Toxicon 39, 1269.  
2. Koo, G.C. et al. (1997) J Immunol. 158, 5120.
3. Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227.
4. Garcia-Calvo, M. et al. (1993) J. Biol. Chem. 268, 18866.
5. Garcia, M. L. et al. (1994) Biochemistry 33, 6834.
6. Koschak, A. et al. (1998) J. Biol. Chem. 273, 2639.
7. Kalman, K. et al. (1998) J. Biol. Chem. 273, 32697.
For research purposes only, not for human use.