Anti-KCa1.1 (BKCa)(1184-1200)


Product#:APC-107

Sizes:
50 ml
0.2 ml


KCa1.1 (BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated  
by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded 
electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it
 can be activated by both an increase in intracellular Ca2+ and by membrane depolarization.  
KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect  
between intracellular Ca2+ signaling pathways and membrane excitability.  
Indeed, KCa1.1 channels have a crucial role in smooth muscle contractility, neuronal spike shaping and  
neurotransmitter release. 

 
The Anti-KCa1.1 antibody is directed against an epitope located at the  
C-terminal intracellular region of the mouse protein. This epitope is located within the epitope of our  
current anti-KCa1.1 (#APC-021) that was made against a GST-fusion protein containing amino acids 1164-1262 of the mouse splice variant 2 (Q08460-2).  
This antibody is directed against a smaller epitope to improve the specificity and sensitivity of the antibody.



Host:
Rabbit.
Epitope:
Peptide (C)STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200 of mouse KCa1.1  
(Accession number Q08460).
Putative epitope location:
Intracellular, C-terminal part.
Homology:
Rat -identical; human, bovine, chicken, dog-16/17 residues identical.
Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
 
Western blotting of rat brain membranes: 
1. Anti-KCa1.1 (BKCa) antibody (1:500). 
2. Anti-KCa1.1 (BKCa) antibody, preincubated with the control peptide antigen.
Immunohistochemistry:
Expression of KCa1.1 in Rat Penis.

Transversal section of the rat penis was stained with Anti-KCa1.1 antibody (#APC-107).  Strong and specific immunostaining is evident in both corpus cavernosum smooth muscle cells (blue arrow) and in the muscular layer of the penis artery (green arrow).  Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Subtrate (histofine, Nichirei Corp.) was used for the color reaction.  Counterstain is Hematoxillin.


References:
1. Hofland L.J. and Lamberts, S.W.J. (2001) Annals of Oncology 12, S31.  
2. Fombonne, J. et al. (2003) Rep. Biol. Endocrinol. 1, 19.
3. Slooter, G.D. et al. (2001) Br. J. Surg. 88, 31.  
4. Schulz, S. et al. (2002) Gynecologic Oncology 84, 235.  
For research purposes only, not for human use.