Anti-KCa2.1 (SK1, SKCa1)


Product#:APC-039

Sizes:
50 ml 
0.2 ml


KCa2.1 is a member of the Ca2+-activated K+ channels family with small conductance that includes  
KCa2.2 (SK2) and KCa2.3 (SK3). The channel is voltage insensitive and is activated by intracellular  
Ca2+ in the submicromolar range. It has a similar topology to that of voltage-dependent  
K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. 

KCa2.1 channel expression is roughly confined to neuronal tissue, although there are some indications  
that it may be present in non-neuronal tissues as well.  

The sensitivity of KCa2.1 to the bee venom toxin Apamin (#A-200) is a matter of some controversy.  
Expression of the human KCa2.1 in mammalian cell lines showed that most cells produce channels
that can be blocked by Apamin. However, expression of KCa2.1 in Xenopus oocytes produced channels  
that were mostly insensitive to apamin. Interestingly, the rat KCa2.1 is unable to form functional channels  
when expressed alone in either cell lines or oocytes. 

The Anti-KCa2.1 antibody (#APC-039) is directed against an  epitope located in the N-terminal intracellular region.


Host:
Rabbit.
Epitope:
Peptide (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCa2.1 
Putative epitope location:
Intracellular, N-terminal part.
Homology:
Mouse -17 out of 19 residues identical, human- 12 out of 19 residues identical.
Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
Western blotting of rat brain cortex.
Western blotting of rat brain cortex:  
1. Anti-KCa2.1 antibody(1:200).  
2. Anti- KCa2.1 antibody, preincubated with the control peptide antigen.

References:
1. Sailer, C.A. et al. (2002) J. Neurosci. 22, 9698.
2. Monaghan, A.S. et al. (2004) J. Biol. Chem. 279, 1003.  
For research purposes only, not for human use.