Anti-Kv10.1 (EAG-1)


Product#:APC-104

Sizes:
50 ml
0.2 ml


KV10.1 is a member of the Ether-a-go-go family of voltage-activated K+ channels. To date, the family includes 8 members: the closely related KV10.2 (EAG-2) protein, the KV12 (elk) subfamily (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). As with the other KV superfamily members, KV10.1 shows the characteristic 6 transmembrane domains with intracelullar N and C termini.

The KV10 subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. Its expression is normally confined to the brain where its physiological function has not yet been clarified.

Intriguingly, the KV10.1 channel has been implicated in malignant tumor development. It has been shown that KV10.1 was inappropriately expressed in several cancer cell lines. Moreover, one study showed that KV10.1 by itself had oncogenic potential, as a cell line transfected with the channel, induced aggressive and faster tumor growth in vivo as compared to a cell line transfected with an unrelated KV channel.

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of rat Kv10.1. The Anti-Kv10.1 (EAG-1) antibody (#APC-104) can be used for western blot and immunocytochemical applications. It has been designed to recognize Kv10.1 from rat, human and mouse samples. The antibody is specific for KV10.1 and it will not crossreact with KV10.2.

Host:

Rabbit.

Type:
Polyclonal.

Epitope:

Peptide GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KV10.1 (Accession Q63472).

Putative epitope location:

Intracellular, C-terminus.

Homology:

Mouse - identical; human -14/16 amino acid residues identical.

Reactivity Confirmed:

Rat, human.

Applications:
Western Blotting:

 

Western blot analysis of rat brain lysate (lanes 1 and 3) and HEK-KV10.1 (lanes 2 and 4):
1, 2. Anti- KV10.1 (EAG-1) antibody (#APC-104), (1:200).
3, 4. Anti-KV10.1 (EAG-1) antibody, preincubated with the control antigen.

Immunocytochemistry:
Expression of Kv10.1 in human MDA-468 mammary gland adenocarcinoma cells.

Immunocytochemical staining of paraformaldehyde fixed and permeabilized human MDA-468 mammary gland adenocarcinoma cells. A. Cells were stained with Anti- Kv10.1 (EAG-1) antibody (#APC-104), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). C. Merged image of panels A and B.


References:
1. Ludwig, J. et al. (1994) EMBO J. 13, 4451.
2. Bauer, C. K. et al. (2001) J. Membrane Biol. 182, 1.
3. Pardo, LA. et al. (1999) EMBO J. 18, 5540.
For research purposes only, not for human use.