Anti-Kir6.1
A Polyclonal Antibody to Kir6.1 (KATP) Inward Rectifier K+ Channel
| Product#: | APC-105 |
| Sizes: |
| 50 ml |
| 0.2 ml |
Kir 6.1 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes.
Kir 6.1 like its close relative Kir 6.2 is highly sensitive to inhibition by intracellular ATP. Closure of the channel leads to membrane depolarization hence coupling intracellular metabolism to cellular excitability.
Kir 6.1 presents the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N and C-terminus located intracellularly.
The functional ATP sensitive channel (KATP) is composed of octamers of four Kir 6.x subunits and four members of the sulfonylurea receptor family SUR1, SUR2A and SUR2B.
Kir 6.1 tissue distribution is relatively broad with expression detected in heart, brain, and smooth muscle.
We are pleased to introduce an antibody against a highly specific region in the C-terminal intracellular domain of the rat Kir 6.1 subunit. Anti-Kir 6.1 antibody (#APC-105) should also recognize Kir 6.1 from human, mouse, pig and rabbit origin.
| Epitope: |
|
Peptide (C)KRNSMRRNNSMRRSN, corresponding to amino acid residues 382-396 of rat K ir6.1 (Accession number Q63664 ). |
|
| Putative epitope location: |
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Intracellular, C-terminal part. |
| Homology: |
|
Mouse, human, pig, rabbit – 15/16 residues identical. Specificity: The antibody is specific for Kir6.1, it does not cross react with Kir6.2. |
| Reactivity Confirmed: |
| Rat. |
| Western Blotting: |
| . |
 | Western blotting of rat heart membranes: 1. Anti-Kir6.1 antibody (1:200). 2. Anti-Kir6.1 antibody (1:200), preincubated with the control peptide antigen. |
 | Western blotting of rat cortex lysate: 1. Anti-Kir6.1 antibody (1:200). 2. Anti-Kir6.1 antibody (1:200), preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
| Longitudinal section of the myometrium in pregnant rat uterus stained with Anti-Kir6.1 antibody (#APC-105) (1:50). Strong and specific staining is evident in smooth muscles cells both in the myometrium (blue arrow) and muscular layers of blood vessels (green arrow). Peroxidase reaction with DAB were used for the color reaction. Counterstain is Hematoxilin. |
| References: |
| 1. Baukrowitz, T. et al. (2000) Eur. J. Biochem. 267, 5842. |
| 2. Aguilar-Bryan, L. et al. (1998) Physiol. Rev. 78, 227. |