Anti-slob4 (KCNMB4)

A Polyclonal Antibody to KCa1 slob4 auxilliary subunit


Product#:APC-061

Sizes:
50 ml
0.2 ml


slob4 is a member of a family of regulatory b subunits that control the activity of the large conductance Ca 2+-activated K+ channel KCa1.1. The family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region. The four members of the family have a distinct tissue distribution with slob4 expressed almost exclusively in the central nervous sytem (CNS).

Functionally, slob4 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology. It has been shown that co-expression of slob4 with KCa1.1 makes the latter resistant to nM concentrations of the well-known inhibitors Charybdotoxin (#RTC-325) and Iberiotoxin (#RTI-400).

The physiological significance of slob4 expression in the CNS is not clear, but KCa1.1 channels are likely involved in the regulation of neurotransmitter release in presynaptic terminals. 

We are pleased to introduce a new antibody directed against the intracellular N-terminus of the rat slob4. Anti-slob4 (KCNMB4) (#APC-061) will also recognize slob4 from human and mouse origin.



Host:
Rabbit.
Epitope:

Peptide AKLRVSYEYTEAEDKS(C), corresponding to amino acid residues 2-17 of rat slob4 (Accession Q9ESK8).

Putative epitope location:

Intracellular, N-terminal part.

Homology:

Mouse, human –identical.

Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
.
Western blotting of rat brain membranes:
1. Anti-slob4 antibody (1:200)
2. Anti- slob4 antibody, preincubated with the control peptide antigen.

References:
1. Meera, P. et al. (2000) PNAS 97, 5562.
2. Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
3. Brenner, R. et al. (2000) J. Biol. Chem. 275, 6453.
For research purposes only, not for human use.