Anti-slob1 (KCNMB1)

A Polyclonal Antibody to KCa1 slob1 auxilliary subunit


Product#:APC-036

Sizes:
50 ml
0.2 ml


slob1 is a member of a family of regulatory b subunits that control the activity of the large conductance Ca2+-activated K+ channel KCa1.1. The family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region. The four members of the family have a distinct tissue distribution with slob1 expressed almost exclusively in smooth muscle.

Functionally, slob1 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology.

In the past few years there has been a lot interest regarding the role of the slob1 subunit in the regulation of vascular tone and hypertension.  Studies in slob1 knockout mice have shown that loss of this subunit results in systemic hypertension. Conversely, a recent study showed that a commonly found gain-of-function slob1 variant has a protective effect against human diastolic hypertension.

We are pleased to present a new antibody directed against the intracellular N-terminus of the rat slob1. Anti-slob1 (KCNMB1) antibody (#APC-036) will also recognize slob1 from human, mouse, dog and rabbit origin.



Epitope:

Peptide KKLVMAQKRGETRALC, corresponding to amino acid residues 2-17 of rat slob1 (Accession P97678).

Putative epitope location:

Intracellular, N-terminal part.

Homology:

Mouse, human, dog, rabbit –identical.

Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
.
Western blotting of rat smooth muscle lysate:
1. Anti-slob1 antibody (1:200).
2. Anti-slob1antibody, preincubated with the control peptide antigen.

References:
1. Knaus, H-G. et al. (1994) J. Biol. Chem. 269, 17274.
2. Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
3. Brenner, R. et al. (2000) Nature 407, 870.
4. Fernández- Fernández, J.M. et al. (2004) J. Clin. Invest. 113, 1032.
For research purposes only, not for human use.