Anti-TRPV4


Product#:ACC-034

Sizes:
50 ml
0.2 ml


TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously
expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence
homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5 TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland.6

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope in the C-terminus of rat TRPV4. Anti-TRPV4 (#ACC-034) can be used for western blot analysis, immunohistochemical and immunocytochemical applications. It has been designed to recognize TRPV4 from human, rat and mouse samples.



Host:

Rabbit.

Type:
Polyclonal.

Epitope:

Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8).

Putative epitope location:

Intracellular, C-Terminus.

Homology:

Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.

Reactivity Confirmed:

Rat.

Applications:
Western Blotting:
 
Western blot analysis of ND7/23 cell line lysate:
1. Anti-TRPV4 antibody (#ACC-034), (1:200).
2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.
Immunohistochemistry:
Expression of TRPV4 in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 antibody (#ACC-034). A. TRPV4 (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.
Immunocytochemistry:
Expression of TRPV4 in rat DRG primary culture

Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture.
A, D. Staining using Anti-TRPV4 antibody (#ACC-034), (1:500), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.

B, E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.

C. Merged image of panels A and B.

F. Merged image of panels D and E.

 


References:
1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
2. Clapham, D.E. (2003) Nature 426, 517.
3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362.
4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183.
6. Peng, J.B. et al. (2003) J. Physiol. 551.3, 729.
For research purposes only, not for human use.