MAPK Homolog - p38 Inhibitor
| Product#: | S-370 |
| Sizes: |
| 10 x 0.1 mg |
| 1 mg |
| 5 x 1 mg |
SB203580 is highly selective and cell permeable pyridinyl imidazole that inhibits the activity of MAP kinase homologs - p38 (stress-activated protein kinase II/HOG1) and p54 (stress-activated protein kinase I/c-Jun N-terminal kinase) with IC50 values of 50 and 500 nM for p38/SAPK2a and p38/SAPK2b respectively. 1-6 SB203580 also inhibits the phosphorylation and activation of protein kinase B (PKB, also known as Akt) by inhibiting the PKB kinase, phosphoinositide-dependent protein kinase 1 (IC50 3-5 µM).7-10
| M.W.: | 377.44 |
| CAS No.: | 152121-47-6 |
| Structure: |
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| Purity: | >99% |
| Bioassay: |
| SB203580 inhibits the phosphorylation of Elk1 and Atf2 caused by p38 MAPK. |
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3T3-L1 cells were grown to 70% confluence and then serum starved for 3 h. The cells were incubated for 2 h with various concentrations of SB203580 and stimulated with 5mM Anisomycin (#A-520). The cell proteins were resolved by SDS PAGE, probed with anti-phospho-p38 MAPK, anti-p38 MAPK, anti-phospho-Elk1 and anti-phospho-Atf2. The phophorylation of Elk1 and Atf2 was reduced as a function of SB203580 concentrations. |
| References: |
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4. |
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Ward, S.G. et al. (1997) Biochem. Soc. Trans. 2, 304S. |
7. |
Ferdinand,V. et al. (2000) J. Biol. Chem. 275, 7395. |
8. |
Lali, F.V. et al. (2000) J. Biol. Chem. 275, 7395. |
9. |
Martin, M. et al. (2003) Infect. Immun. 71, 2498. |
10. |
Darveau, R.P. et al. (2002) Infect. Immun. 70, 1867. |