Anti-Kv1.3


Product#:APC-002

Sizes:
50 µl
0.2 ml


Kv1.3 belongs to the Shaker family of voltage-dependent K+ channels. 
The channel is widely expressed in the brain, lung and osteoclasts and in several cell  
populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that  
Kv1.3 function has centered a lot of attention. It was found that Kv1.3 is the main channel  
responsible for maintaining the resting potential in quiescent cells and regulating the  
Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2  
Based on the central role of Kv1.3 in regulating the initiation of an immune response,  
the channel has been recognized as a potential target for immunosuppressant drugs.1 

KV1.3 channels are potently inhibited by several venomous peptide toxins among them
Charybdotoxin (#RTC-325, 2.6nM), Noxiustoxin (#RTN-340, 1nM), Kaliotoxin (#RTK-370, 0.65nM),
Margatoxin (#RTM-325, 0.05nM), Agitoxin-1 (#RTA-150, 1.7nM), Agitoxin-2 (#RTA-420, 0.004nM),
Hongotoxin-1 (#RTH-400, 0.09nM)  and Stichodactyla toxin (#S-400, 0.01nM).3-7



Host:

Rabbit.

Type:
Polyclonal.

Epitope:

GST fusion protein with sequence TLSKSEYMVIEEGGMNHSAFPQTPFKTGNS TATCTTNNNPNSCVNIKKIFTDV, corresponding to amino acid residues 523-575 of human Kv1.3 (Accession P22001).

Putative epitope location:
Intracellular, C-terminus.
Homology:

Rat, rabbit, mouse - identical.

Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
 

Western blot analysis of rat brain membranes:

1. Anti-Kv1.3 antibody (#APC-002), (1:200)

2. Anti-Kv1.3 antibody, preincubated with the control peptide antigen.

 

Immunohistochemistry:

Rat brain sections


References:
1. Chandy, K.G.et al. (2001) Toxicon 39, 1269.
2. Koo, G.C. et al. (1997) J Immunol. 158, 5120.
3. Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227.
4. Garcia-Calvo, M. et al. (1993) J. Biol. Chem. 268, 18866.
5. Garcia, M. L. et al. (1994) Biochemistry 33, 6834.
6. Koschak, A. et al. (1998) J. Biol. Chem. 273, 2639.
7. Kalman, K. et al. (1998) J. Biol. Chem. 273, 32697.
For research purposes only, not for human use.