Anti-Kv1.5


Product#:APC-004

Sizes:
50 µl
0.2 ml


KV1.5 is a mammalian voltage dependent K+ channel, homologous to the Drosophilae Shaker K+ channel. KV1.5 was first cloned from rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2

A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.

The structure of KV1.5 channel is similar to all KV channels and includes six membrane spanning helixes creating a voltage sensor domain and a pore domain. 2

The channel is expressed in cardiac and smooth muscle tissue (Colon, Aorta, Stomach and Pulmonary artery) as well as in neurons and Kidney.2 A loss of function mutation in the gene encoding the channel was found in atrial fibrilation patients, stressing its role as a cardiac action potential regulator.3

 

The functional channel is considered transient (A-type) channel and shows prominent inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.

 

KV1.5 channels are sensitive to high doses of TEA (330 mM) and low doses of 4-AP (0.27 mM), the “classical” non-selective potassium channel blockers.2

 



Host:

Rabbit.

Type:
Polyclonal.

Epitope:

GST fusion protein with sequence HRETDHEEQAALKEEQGIQRRESGLDTGGQ RKVSCSKASFHKTGGPLEST DSIRRGSCPLEKCHLKAKSNVDLRRSLYALCLDTS RETDL, corresponding to amino acid residues 513-602 of mouse Kv1.5 (Accession Q61762).

Putative epitope location:
Intracellular, C-terminus
Homology:

Rat - 86/90 amino acid residues identical; rabbit - 71/90 amino acid residues identical; human - 70/90 amino acid residues identical; bovine, dog - 66/90 amino acid residues identical.

Reactivity Confirmed:
Rat.
Applications:
Western Blotting:
 

Western blot analysis of rat brain membranes:

1. Anti-Kv1.5 antibody (#APC-004), (1:100).

2. Anti-Kv1.5 antibody, preincubated with the control peptide antigen.

 

Immunohistochemistry:

Rat brain sections.


References:
1. Swanson, R. et al. (1990) Neuron 4, 929.
2. Gutman, G. A. et al. (2005) Pharmacol. Rev. 57, 473.
3. McGahon, M. K. et al. (2007) Am. J. Physiol. Heart Circ. Physiol. 292, H1001.
For research purposes only, not for human use.