Anti-Kvb2


Product#:APC-117

Sizes:
50 ml
0.2 ml


Kvb2 (or KCNAB1) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. The other members of the family are Kvb1 and Kvb3.

The Kvb subunits were originally identified using a biochemical approach that demonstrated that the ion-conducting a subunits existed in a macromolecular complex with auxiliary b subunits with a probable stoichiometry of a4b4. It is now widely established that the regulatory b subunits are able to alter both the biophysical (i.e. acceleration of inactivation kinetics) and biochemical (promote cell surface expression) properties of the functional Kv channel.

The Kvb regulatory subunits are cytosolic proteins with conserved C-termini and variable N-terminus domains. The interaction with the a subunits is via the conserved C-terminal end that binds to a specific sequence in the N-terminus domain of the a subunits.

Kvb2, as well as the other Kvb subunits can bind to all the members of the large Kv1.x (Shaker) channel subfamily.  However, recent evidence suggests that it can also bind to members of the Kv4.x subfamily.

Kvb2 is the most abundant b subunit in the brain where it couples with all the Kv1.x subunits, but the protein is also expressed in peripheral tissues including heart, lung and cells of hematopoietic origin such as T cells where it couples with Kv1.3, the dominant voltage-dependent K+ channel in these cells.

We have now developed a highly specific antibody directed against a unique region in the N-terminus end of the rat Kvb2 protein. The antibody will also recognize Kvb2 samples from mouse and human origin.



Host:
Rabbit.
Epitope:
Peptide SPGMIYSTRYGSPKR(C), corresponding to amino acid residues 20-34 of rat Kvb2 (Accession P62483).
Putative epitope location:

N-terminal part.

Homology:

Mouse, human, rabbit- identical.

Reactivity Confirmed:
Rat, human, mouse.
Applications:
Western Blotting:
 

Western blotting of rat brain lysate (lanes 1 and 3) and membranes (lanes 2 and 4):

1, 2.  Anti-Kvb2 antibody (1:200).
3, 4.  Anti-Kvb2 antibody, preincubated with the control peptide antigen.

Western blotting of human Jurkat T cells:

1.  Anti-Kvb2 antibody (1:200).
2.  Anti-Kvb2 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:
 

Immunohistochemical staining of Kvb2 with Anti-Kvb2 antibody (#APC-117) in mouse cerebellum.

 

  1. Kvb2 appears adjacent to Purkinje cells (green).
  2. Staining of GABAergic cells with mouse anti parvalbumin (PV, red).
  3. Confocal merge of Kvb2 and PV demonstrates presence of Kvb2 adjacent to Purkinje cells.

 


References:
1. Parcej, D.N. et al.  (1992) Biochemistry 31, 11084.
2. Rettig, J. et al. (1994) Nature 369, 289.
3. Pongs, O. et al. (1999) Ann. N. Y. Acad. Sci. 868, 344.
4. Li, Y. et al. (2006) Neuroscientist 12, 199.
For research purposes only, not for human use.