KCNE3 (or MiRP2) is a member of a family of proteins that regulate the activity of voltage-dependent K⁺ channels. The other members of the family are KCNE1( IsK, MiNK), KCNE2 (MiRP1), KCNE4 (MiRP3) and KCNE5 (MiRP4). KCNE1 is the founding member of the family and was initially believed to form a K⁺ channel itself, but was latter recognized that it worked as a regulatory b subunit associated with the K_v7.1 (KCNQ1) a protein. KCNE3 was discovered based on its homology with KCNE1. 

The KCNE regulatory subunits are small proteins (14- 20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the a subunits. The stoichiometry of the KCNE subunits with their partner a subunits in the native channels is not clear and ratios ranging from 2 to 14 KCNE subunits per 4 a subunits have been proposed.

KCNE3 is relatively widely expressed in several tissues with prominent expression in the kidney and skeletal muscle.

KCNE3 is quite promiscuous and associations with K_v7.1, K_v3.4, K_v7.4 (KCNQ4), K_v11.1 (HERG), K_v2.1 and K_v3.1b have been demonstrated. The best characterized interactions are with the former two proteins. KCNE3 interacts with K_v7.1 in epithelial cells of the gastrointestinal tract where it appears to be important for Na⁺ absorption.  In skeletal muscle KCNE3 couples to K_v3.4 to regulate muscle function. Indeed, a mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis).

We have now developed a highly specific antibody directed against the intracellular C-terminal part of the human KCNE3 protein. The antibody will also work with rat and mouse samples.