| Product#: | ACC-064 |
| Sizes: |
| 50 µl |
| 0.2 ml |
Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research.
Recently, several key players of the store-operated complex have been identified: the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4
While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5 The function of STIM2 still remains elusive and several models have been suggested.6-8
Alomone Labs is pleased to offer a highly specific antibody directed against human STIM2. Anti-STIM2 antibody (#ACC-064) can be used in western blot, immunocytochemical, immunohistochemical applications. It has been designed to recognize STIM2 from human, rat, mouse and bovine samples.
| Host: |
Rabbit. Type: |
| Epitope: |
Peptide SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246). |
|
| Putative epitope location: |
Intracellular, C-terminus. |
| Homology: |
Rat, mouse and bovine-identical. |
| Reactivity Confirmed: |
| Rat. |
| Western Blotting: |
 | Western blot analysis of rat brain (lanes 1,3) and RBL cell (lanes 2,4) lysates: 1. Anti-STIM2 antibody (#ACC-064), (1:200). 2. Anti-STIM2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of STIM 2 in rat lung |
 |
Immunohistochemical staining of paraffin embedded rat lung sections using Anti-STIM2 antibody (#ACC-064), (1:100). Staining is present in the respiratory epithelium of the bronchiole (red) as well as in the pneumonocytes of the alveolar wall (blue). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain. |
| Immunocytochemistry: |
| Expression of STIM2 in RBL cells |
 |
Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat basophilic leukemia (RBL) cells. A, D. Cells stained with Anti-STIM2 antibody (#ACC-064), (1:100) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B, E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B F. Merged image of panels D and E. |
| References: |
| 1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. |
| 2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. |
| 3. Feske, S. et al. (2006) Nature 441, 179. |
| 4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol. 7, 794. |
| 5. Dziadek, M.A. and Johnstone, L.S. (2007) Cell Calcium 42, 123. |
| 6. Parvez, S. et al. (2007) FASEB J. 22, 752. |
| 7. Brandman, O. et al. (2007) Cell 131, 1327. |
| 8. Soboloff, J. et al. (2006) Curr. Biol. 16, 1465. |