Anti-Melanocortin Receptor 2 (extracellular)

ACTH Receptor


Product#:AMR-022

Sizes:
50 µl
0.2 ml


Melanocortin Receptor 2 (MC2R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

 

The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of a-, b- and g-melanocyte stimulating hormone (a, b, g-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3

 

One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4

 

All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.

 

MC2R can be also described as the ACTH receptor as it is exclusively activated by the ACTH hormone. In fact, MC2R mediates all the physiological effects of ACTH in the adrenal cortex, that is the regulation and production of steroids, their circadian variation and their stress-related fluctuations.1, 2, 3 In addition, mutations that result in MC2R functional impairment are associated with familial glucocorticoid deficiency.4

 

The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP. 

 

Alomone Labs is pleased to offer a highly specific antibody directed against the extracellular 1st loop of the human MC2R. This antibody can be used in Western blot and immunohistochemistry applications and will recognize human, mouse and rat samples.



Host:
Rabbit.
Epitope:
Peptide (C)RNMGYLKPRGSFE  corresponding to amino acid residues 87-99 of human MC2R (Accession Q01718).
Putative epitope location:
Extracellular, 1st  loop.
Homology:

Rat, mouse- identical.

Applications:
Western Blotting:
 

Western blot analysis of rat adrenal lysate:

  1. Anti-MC2R (extracellular) antibody (#AMR-022) (1:400).
  2. Anti-MC2R (extracellular) antibody, preincubated with the control peptide antigen.
Immunohistochemistry:
Immunohistochemical staining of MC2R in rat adrenal gland.

Paraffin embedded section of rat adrenal cortex showing the capsule (C), the zona glomerulosa (ZG) and the zona fasciculata (ZF) of the adrenal cortex. Note that stain (brown) is specific for cells of the adrenal gland while no staining is present in the dense connective tissue of the capsule.

After citrate treatment in a pressure cooker for antigen retrieval, slides were incubated overnight at 4°C with Anti-MC2R (extracellular) antibody (#AMR-022) (1:100). Color reaction was obtained with DAB. Counterstain is H&E.


References:
1. Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434.
2. Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. Endocrinol. Metab. 284, E468.
3. Wikberg, J.E.S. et al. (2000) Pharm. Res.42, 393.
4. Elias, L.L. et al. (1999) J Clin Endocrinol Metab 84, 2766.
For research purposes only, not for human use.