| Product#: | ARR-002 |
| Sizes: |
| 50 µl |
| 0.2 ml |
It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.
The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3
The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.
RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain. 1-4
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the cytoplasmic region of the human Ryanodine receptor 2 (RyR2). The Anti-Ryanodine Receptor 2 antibody (#ARR-002) can be used for Western blot analysis and Immunohistochemical applications. It has been designed to recognize RyR2 from human, rat and mouse samples.
| Host: |
Rabbit.
Type: Polyclonal. |
| Epitope: |
Peptide CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human Ryanodine Receptor 2 (Accession Q92736). |
|
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
Rat, mouse - identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
| Western Blotting: |
 | Western blot analysis of rat heart membranes: 1. Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:200). 2. Anti-Ryanodine Receptor 2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
|
Expression of Ryanodine Receptor 2 in rat cardiac muscle |
 |
Immunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain. |
 |
Immunohistochemical staining of mouse cerebellum frozen sections with Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:100), (green fluorescence). A. The highest expression of Ryanodine Receptor 2 is in the molecular layer (Asterisk) but there is also some expression in the soma of Purkinje cells (arrows). B. In the same section, there is staining for parvalbumin (red), a marker for Purkinje cells. C. Merged image of panels A and B demonstrates that Ryanodine Receptor 2 is localized both in the area surrounding the dendritic tree and in the soma of Purkinje cells. DAPI is used as the counterstain (blue). |
| References: |
| 1. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3. |
| 2. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503. |
| 3. Fill, M. and Copello, J.A. (2002) Physiol Rev. 82, 893. |
| 4. Bers DM. (2004) J.Mol. Cell. Cardiol. 37, 417. |