| Product#: | APC-122 |
| Sizes: |
| 50 µl |
| 0.2 ml |
K2P18.1 (also named TWIK-related spinal cord K+ channel, TRESK or KCNK18) is a member of the 2-pore (2P) domain K+ channels family that in mammals includes 15 members. These channels show little time or voltage dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1
The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. It has been proposed that the functional channel unit is a dimer.
Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.
K2P18.1 is the only K2P channel so far, whose current is activated following Gaq-receptor coupled activation. The enhancement of K2P18.1 current involves activation of calcineurin (calcium–calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gaq activation.2 In addition, K2P18.1 is potently activated by clinical concentrations of volatile anesthetics.3
K2P18.1 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.
K2P18.1 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.
Alomone labs is pleased to offer a highly specific Anti-K2P18.1 (TRESK)(extracellular) (#APC-122) antibody directed against a well conserved epitope located in the first extracellular region of the mouse K2P18.1 channel.
Anti-K2P18.1 (TRESK)(extracellular) (#APC-122) antibody can be used in Western blot, immunohistochemical, and immunocytochemical applications and will recognize K2P18.1 from rat and mouse samples.
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse K2P18.1 (Accession Q6VV64 ). |
 |
| Putative epitope location: |
Extracellular, 1st loop. |
| Homology: |
Rat – 13/14 residues identical. Unlikely to recognize human samples. |
| Reactivity Confirmed: |
Rat, mouse. |
| Western Blotting: |
 | Western blot analysis of rat brain (lanes 1 and 3) and dorsal root ganglion (lanes 2 and 4) membranes: 1, 2. Anti-K2P18.1 (extracellular) antibody (#APC-122) (1:200). 3, 4. Anti-K2P18.1 (extracellular), preincubated with the control peptide antigen. |
 | Western blot analysis of mouse brain membranes:
|
| Immunohistochemistry: |
 |
| Expression of K2P18.1 in rat dorsal root ganglia. Immunohistochemical staining of frozen sections of rat dorsal root ganglia (DRG) using Anti- K2P18.1 (TRESK) antibody (#APC-122), (1:100) followed by Alexa 555-labeled secondary antibody (red staining). Both big/medium sized neurons and small neurons are stained. Note that glial cells and axonal fibers are not stained. Hoechst 33342 is used as the counterstain (blue staining). |
| Immunocytochemistry: |
 |
Immunocytochemical staining of K2P18.1 channel in a mouse/rat neuroblastoma x dorsal root ganglion neuron hybrid cell line. A. Live intact ND7/23 (neuroblastoma x dorsal root ganglion) hybrid cells were stained with Anti- K2P18.1 (extracellular) antibody (#APC-122) (1:50), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). |
| References: |
| 1. Goldstein, S.A.N. et al. (2003) Nat. Rev. Neurosci. 2, 175. |
| 2. Mathie, A. (2007) J. Physiol. 578, 377. |
| 3. Liu, C. et al. (2004) Anesth. Analg. 99, 1715. |