Anti-Angiotensin-(1-7) Mas Receptor


Product#:AAR-013

Sizes:
50 µl
0.2 ml


The angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide angiotensin-(1-7).1

 

Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis.

 

Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3

 

The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene.

Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3

 

The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2

 

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 3rd intracellular loop of the rat Ang-(1-7) Mas receptor. Anti-Angiotensin-(1-7) Mas receptor antibody (#AAR-013) can be used in Western blot and immunohistochemical applications, and recognizes Ang-(1-7) Mas receptor from rat, mouse and human samples.



Host:
Rabbit.
Epitope:

Peptide (C)KIRKNTWASHSSK corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor (Accession P12526).

Putative epitope location:

Intracellular, 3rd loop

Homology:

Mouse, human- identical.

Reactivity Confirmed:

Rat, mouse, human.

Applications:
Western Blotting:
 

Western blotting of rat brain (lanes 1 and 4) kidney (lanes 2 and 5) and heart (lanes 3 and 6) membranes:

1, 2, 3.  Anti- Ang-(1-7) Mas receptor antibody (AAR-013), 1:200.

4, 5, 6.  Anti- Ang-(1-7) Mas receptor, preincubated with the control peptide antigen.

Western blotting of mouse kidney membranes:

1.  Anti- Ang-(1-7) Mas receptor antibody (AAR-013), 1:200.

2.  Anti- Ang-(1-7) Mas receptor, preincubated with the control peptide antigen.

Western blotting of human HeLa cervix adenocarcinoma cell line lysate:

1.  Anti- Ang-(1-7) Mas receptor antibody (AAR-013), 1:200.

2.  Anti- Ang-(1-7) Mas receptor, preincubated with the control peptide antigen.

Immunohistochemistry:
Expression of Angiotensin-(1-7) Mas receptor in rat kidney.

Immunohistochemical staining of paraffin embedded sections of rat kidney using Anti-Angiotensin-(1-7) Mas receptor antibody (#AAR-013) (1:100).

Ang-(1-7) Mas receptor is visualized (brown staining) in proximal tubules (PT) and distal tubules (DT) in the renal cortex. Note that collecting ducts (CD) are less stained and both glomeruli (G) and blood vessels (A) are negative.  Counterstain is Hematoxilin.


References:
1. Santos, R.A.S. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 8258.
2. Alenina, N. et al. (2008) Exp. Physiol. 93, 528.
3. Santos, R.A.S. et al. (2008) Exp. Physiol. 93, 519.
For research purposes only, not for human use.