Anti-Kir5.1


Product#:APC-123

Sizes:
50 µl
0.2 ml


Kir5.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.

 

The family’s protein topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assembly as either homo or heteromers.

 

Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.1

 

Kir5.1 can co-assemble with other Kir channels such as Kir2.1, Kir4.1 and Kir4.2.  In fact, heteromeric Kir4.1/ Kir5.1 form the basolateral small-conductance K+ channel in distal nephron segments that is responsible for generating the basolateral membrane potential that determines the magnitude and direction of K+ diffusion from cell to peritubular fluid in the nephron.2

 

The Kir5.1 homomeric channel was largely thought to be non-functional, although recent evidence demonstrates that Kir5.1 homomers can form functional channels when co-expressed with the anchor protein PSD-95 in the brain where it is abundantly expressed.3

 

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of the rat Kir5.1 channel. Anti-Kir5.1 antibody (#APC-123) can be used in Western blot and immunohistochemical applications, and was designed to recognize Kir5.1 from rat, mouse and human samples.



Host:
Rabbit.
Epitope:

Peptide HDVLEVKRKYYKVNC, corresponding to amino acid residues 311-325 of rat Kir5.1 (Accession P52191)

 
Putative epitope location:

Intracellular, C-term.

Homology:

Mouse- identical; human 14 out of 15 residues-identical.

 

Reactivity Confirmed:

Rat, mouse.

Applications:
Western Blotting:
 

Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes:

1, 2.  Anti- Kir5.1 antibody (#APC-123) (1:200).

3, 4.  Anti-Kir5.1, preincubated with the control peptide antigen.

Western blot analysis of mouse brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:

1, 2.  Anti- Kir5.1 antibody (#APC-123) (1:200).

3, 4.  Anti-Kir5.1, preincubated with the control peptide antigen.

Immunohistochemistry:
Expression of Kir5.1 in rat kidney.

Immunohistochemical staining of paraffin embedded section of rat kidney using Anti- Kir5.1 antibody (#APC-123) (1:100). Kir5.1 (brown staining) is expressed in both proximal tubules (PT) and distal tubules (DT) in the renal cortex. Note that collecting ducts (CD) are less stained while glomeruli (G) are negative.

Counterstain is Hematoxilin.


References:
1. Bond, C.T. et al. (1994) Rec. Channels 2, 183.
2. Hebert, S.H. et al. (2005) Physiol. Rev. 85, 319.
3. Tanemoto, M. et al. (2002) Neuron 34, 387.
For research purposes only, not for human use.