| Product#: | ACC-029 |
| Sizes: |
| 50 µl |
| 0.2 ml |
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1
According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5
TRPV1 channel has many activators among them heat, protons, vanilloids like capsaicin, reiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7
TRPV1 is expressed predominantly in nociceptors and in sensory neurons.
Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel. Anti-Rat TRPV1 (extracellular) antibody (#ACC-029) can be used for western blot and immunohistochemistry applications. It has been designed to recognize only rat TRPV1 samples.
| Host: | ||
Rabbit.
Polyclonal. |
| Epitope: |
Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433). |
|
| Putative epitope location: |
| 3rd extracellular loop. |
| Homology: |
Rat. Specificity: |
| Reactivity Confirmed: |
| Rat. |
| Western Blotting: |
 | Western blot analysis of TRPV1 in rat DRG: 1. Anti-Rat TRPV1 (extracellular) antibody (#ACC-029), (1:200). 2. Anti-Rat TRPV1 (extracellular) antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of TRPV1 in rat DRG |
 |
Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Rat TRPV1 (extracellular ) antibody (#ACC-029), (1:100) followed by goat anti-rabbit-ATTO-594 secondary antibody (red). TRPV1 is expressed in medium and small DRG neurons. Counterstain is Hoechst 33342 (blue). |
| References: |
| 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. |
| 2. Clapham, D.E. (2003) Nature 426, 517. |
| 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. |
| 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. |
| 5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183. |
| 6. Ross, R.A. et al. (2003) Br. J. Pharmacol. 140, 790. |
| 7. Tominaga, M. et al. (1998) Neuron 21, 531. |
| 8. Agopyan, N. et al. (2004) Am. J. Physiol .Lung .Cell .Mol. Physiol. 286, 563. |
| 9. Reilly, |