Anti-Rat TRPV1 (extracellular)-ATTO-488


Product#:ACC-029-AG

Sizes:
50 µl


TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1

According to IUPHAR the TRP family comprises three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

TRPV1 channel has many activators; among them heat, protons, vanilloids like capsaicin, reiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7

TRPV1 is expressed predominantly in nociceptors and in sensory neurons.

Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

 

Alomone Labs is pleased to offer a new version of the Anti-rat-TRPV1 (extracellular)antibody (#ACC-029) that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. The Anti-Rat-TRPV1 (extracellular)-ATTO-448 antibody (#ACC-029-AG) has been tested in immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.

 



Host:

Rabbit.

 

Type:

Polyclonal.

Epitope:

Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433).

Putative epitope location:
3rd extracellular loop.
Homology:

Specific to rat TRPV1.

 

 Label:

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range.

This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Reactivity Confirmed:

Rat.

 

Standard Quality Control of each lot:
Western blot analysis (unlabeled antibody, see datasheet of product #ACC-029) and immunohistochemistry (labeled antibody).

Applications:
Immunohistochemistry:

Expression of TRPV1 in rat DRG

Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Rat TRPV1 (extracellular )-ATTO-488 antibody (#ACC-029-AG), (1:50), (green). TRPV1 is expressed in medium and small DRG neurons.

Hoechst 33342 is used as the counterstain.

 


References:
1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
2. Clapham, D.E. (2003) Nature 426, 517.
3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362.
4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183.
6. Ross, R.A. et al. (2003) Br. J. Pharmacol. 140, 790.
7. Tominaga, M. et al. (1998) Neuron 21, 531.
8. Agopyan, N. et al. (2004) Am. J. Physiol .Lung .Cell .Mol. Physiol. 286, 563.
9. Reilly, C.A. et al. (2003) Toxicol. Sci. 73, 170.
For research purposes only, not for human use.