Anti-TRPV4-ATTO-550


Product#:ACC-034-AO

Sizes:
50 µl


TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously
expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence
homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65. TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.

Alomone Labs offers a highly specific antibody directed against an intracellular epitope of TRPV4. Anti-TRPV4 antibody (#ACC-034) can be used in Western blot, immunocytochemical, and immunohistochemical applications and recognizes TRPV4 from rat samples.

Alomone Labs is pleased to offer a new version of this antibody, Anti-TRPV4-ATTO-550 antibody (#ACC-034-AO), that is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-TRPV4-ATTO-550 antibody has been tested in immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.



Host:

Rabbit.

Host:
Polyclonal.

Epitope:
Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8).
Putative epitope location:

Intracellular, C-Terminus.

Homology:

Mouse - 17/19 amino acid residues identical; human 16/19 amino acid residues identical.

Label:
ATTO-550. Maximun absorption 554 nm; maximum fluorescence 576 nm.  The fluorescence is excited most efficiently in the range of 540-565 nm. This label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine.

Reactivity Confirmed:

Rat.

Standard Quality Control of each lot:
Western blot analysis (unlabeled antibody, see datasheet of product #ACC-034) and immunohistochemistry (labeled antibody).

 

Applications:
Immunohistochemistry:
Expression of TRPV4 in rat DRG
Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections with Anti-TRPV4-ATTO-550 antibody (#ACC-034-AO), (1:20). TRPV4 is present in DRG neurons (red). Note that staining is stronger in small cells (arrows). Hoechst 33342 is used as the counterstain. 

References:
1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
2. Clapham, D.E. (2003) Nature 426, 517.
3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362.
4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183.
6. Peng, J.B. et al. (2003) J. Physiol. 551.3, 729.
For research purposes only, not for human use.