Anti-TRPM2

Product#:

ACC-043

Sizes:

50 µl

0.2 ml

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas^1-3.

The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7^1,2.

The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family^1-3.

The C-terminal regions of three TRPM members (TRPM2, TRPM 6 and TRPM 7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates^2,4.

TRPM2 is a Ca²⁺-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified^5,3.

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse TRPM2. The Anti-TRPM2 antibody (#ACC-043) can be used for western blot and immunohistochemical applications. It has been designed to recognize TRPM2 from mouse, rat and human samples.

Host:

Rabbit.

Type:

Polyclonal.

Epitope:

Peptide (C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2 (Accession Q91YD4).

Putative epitope location:

Intracellular, N-terminus.

Homology:

Rat – 15/16 amino acid residues identical; human – 14/16 amino acid residues identical.

Reactivity Confirmed:

Rat, human.

Applications:

Western Blotting:

Western blot analysis of TRPM2 in rat lung membrane (lanes 1 and 4), rat brain membrane (lanes 2 and 5) and human K562 cells (lanes 3 and 6) lysates:

1-3. Anti-TRPM2 antibody (#ACC-043), (1:200).

4-6. Anti-TRPM2 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:

Expression of TRPM2 in rat lung

Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM2 antibody (#ACC-043), (1:100). TRPM2 is expressed in the respiratory epithelium (black arrows). In addition staining is also present in smooth muscle, both bronchiolar (red arrowheads) and vascular (red arrows). Hematoxilin is used as the counterstain.

References:

1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633.

2. Kraft, R. and Harteneck, C. (2005) Pflugers Arch – Eur. J. Physiol. 451, 204.

3. Huang, C-L. et al. (2004) J. Am. Soc. Nephrol. 15, 1690.

4. Montell, C. (2005) Sci. STKE. 2005, re3.

5. Du, J. et al. (2009) Proc. Natl. Acad. Sci. USA. 106, 7239.

For research purposes only, not for human use.