Anti-TRPM4

Product#:

ACC-044

Sizes:

50 µl

0.2 ml

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas^1-3.

The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7^1,2.

The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family^1-3.

TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca²⁺.

TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca²⁺ concentration⁴.

TRPM4 is expressed at relatively high levels in cardiac tissues.

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human TRPM4. The Anti-TRPM4 antibody (#ACC-044) can be used for western blot and immunohistochemical applications. It has been designed to recognize TRPM4 from human, rat and mouse samples.

Host:

Rabbit.

Type:

Polyclonal.

Epitope:

Peptide EKEQSWIPKIFKK (C), corresponding to amino acid residues 5-17 of human TRPM4 (Accession Q8TD43).

Putative epitope location:

Intracellular, N-terminus.

Homology:

Rat, mouse – 12/13 amino acid residues identical.

Reactivity Confirmed:

Rat, mouse.

Applications:

Western Blotting:

Western blot analysis of TRPM4 in rat lung (lanes 1 and 5), rat heart membrane (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and ms1 cells lysate (lanes 4 and 8):

1-4. Anti-TRPM4 antibody (#ACC-044), (1:200).

5-8. Anti-TRPM4 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:

Expression of TRPM4 in rat lung

Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM4 antibody (#ACC-044), (1:100). TRPM4 is expressed in the respiratory epithelium of the bronchioli (arrows).

Hematoxilin is used as the counterstain.

References:

1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633.

2. Kraft, R. and Harteneck, H. (2005) Pflugers Arch – Eur. J. Physiol. 451, 204.

3. Huang, C-L. et al. (2004) J. Am. Soc. Nephrol. 15, 1690.

4. Vazquez, E. and Valverde, M.A. (2006) Semin. Cell Dev. Biol. 17, 607.

For research purposes only, not for human use.