Anti-TRPM5


Product#:

ACC-045


Sizes:

50 µl

0.2 ml



The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. 

The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.

The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. 

TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+.

TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4,5.

 

TRPM5 is primarily expressed in taste buds and also in stomach, small intestine, uterus and testes2.

 

Regulation of TRPM5 by G-protein-coupled receptors and PLC-mediated IP3 production has been substantiated by the colocalization of TRPM5, of a taste-cell-specific PLC isoform and of receptors for sweet, bitter and amino acid (umami) tastes in taste tissue. It has furthermore been shown that desensitization of TRPM5 following receptor stimulation can be reversed by phosphatidylinositol (4,5)-bisphosphate (PIP2), the substrate of PLC2.

 

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse TRPM5. The Anti-TRPM5 antibody (#ACC-045) can be used for western blot and immunohistochemical applications. It has been designed to recognize TRPM5 from mouse, rat and human samples.

 



Host:

Rabbit.

Type:
Polyclonal.

Epitope:

Peptide GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5 (Accession Q9JJH7).

Putative epitope location:

Intracellular, N-Terminus.

Homology:

Rat, human - 13/14 amino acid residues identical.

Reactivity Confirmed:

Rat, mouse, human.

Applications:
Western Blotting:
 

Western blot analysis of TRPM5 in rat lung membrane (lanes 1 and 2), rat testes (lanes 3 and 4), mouse ms1 cells (lanes 5 and 7) and human LNCaP cell (lanes 6 and 8) lysates:

1, 3, 5, 6. Anti-TRPM5 antibody (#ACC-045),(1:200).

2, 4, 7, 8. Anti-TRPM5 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:

Expression of TRPM5 in rat tongue

Immunohistochemical staining of rat tongue paraffin embedded sections using Anti-TRPM5 antibody (#ACC-045), (1:100). TRPM5 is expressed in discrete groups of cells deep in the sulcus of the papillae. Hematoxilin is used as the counterstain.

 

Expression of TRPM5 in rat testes

Immunohistochemical staining of rat epididymis paraffin embedded sections using Anti-TRPM5 antibody (#ACC-045), (1:100). TRPM5 is expressed in principal cells within the epididymal epithelium (arrows). Hematoxilin is used as the counterstain.


References:
1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633.
2. Vazquez, E. and Valverde, M.A. (2006) Semin. Cell Dev. Biol. 17, 607.
3. Kraft, R. and Harteneck, C. (2005) Pflugers Arch – Eur. J. Physiol. 451, 204.
4. Fang, D. and Setaluri, V. (2000) Biochem Biophys Res Commun. 279, 53.
5. Huang, C-L. et al. (2004) J. Am. Soc. Nephrol. 15, 1690.
6. Montell, C. (2005) Sci. STKE.  2005, re3.
For research purposes only, not for human use.