Anti-TRPM2-ATTO-594

Product#:

ACC-043-AR

Sizes:

50 µl

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas^1-3.

The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7^1,2.

The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family^1-3.

The C-terminal regions of three TRPM members (TRPM2, TRPM 6 and TRPM 7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates^2,4.

TRPM2 is a Ca²⁺-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified^5,3

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse TRPM2 that is directly labeled with an ATTO-594 fluorescent dye, Anti-TRPM2-ATTO-594 antibody (#ACC-043-AR). ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. The Anti-TRPM2-ATTO-594 antibody can be used for immunohistochemical applications. It has been designed to recognize TRPM2 from mouse, rat and human samples.

Host:

Rabbit.

Type:

Polyclonal.

Epitope:

Peptide (C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2 (Accession Q91YD4).

Putative epitope location:

Intracellular, N-terminus.

Homology:

Rat – 15/16 amino acid residues identical; human – 14/16 amino acid residues identical.

Label:

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range.

This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Reactivity Confirmed:

Human, rat.

Standard Quality Control of each Lot:
Western blot analysis (unlabeled antibody), see datasheet of product (#ACC-043) and immunohistochemistry (labeled antibody).

Applications:

Immunohistochemistry:

Expression of TRPM2 in rat lung

Immunohistochemical staining of rat lung frozen sections using Anti-TRPM2-ATTO-594 antibody (#ACC-043-AR), (1:50).

A. Staining is specific for respiratory epithelium in the bronchi.

B. Merged with bright field.

References:

1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633.

2. Kraft, R. and Harteneck, C. (2005) Pflugers Arch – Eur. J. Physiol. 451, 204.

3. Huang, C-L. et al. (2004) J. Am. Soc. Nephrol. 15, 1690.

4. Montell, C. (2005) Sci. STKE. 2005, re3.

5. Du, J. et al. (2009) Proc. Natl. Acad. Sci. USA. 106, 7239.

For research purposes only, not for human use.