Anti-TRPC3-ATTO-594


Product#:

ACC-016-AR


Sizes:

50 µl



The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel family and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4

The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and
functional similarities:

TRPC1; TRPC4 and TRPC5; TRPC3, TRPC6, TRPC7 and TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in
peripheral tissues.

TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.

TRPC3 was shown to be activated by DAG and SOC, in heterologous expression
systems.6,7

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse TRPC3 directly labeled with ATTO-594 fluorescent dye, Anti-TRPC3-ATTO-594 antibody (#ACC-016-AR). ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. The Anti-TRPC3-ATTO-594 antiobdy can be used for immunohistochemical application. It has been designed to recognize TRPC3 from mouse, rat and human.

 



Host:

Rabbit.

Type:

Polyclonal.

Epitope:

Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1).

Putative epitope location:

Intracellular, C-terminus.

Homology:

Human, rat - 13/14 amino acid residues identical.

Label:

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Reactivity Confirmed:

Rat.

Standard Quality Control of each lot:

Western blot analysis (unlabeled antibody, see datasheet of product #ACC-016) and immunohistochemistry (labeled antibody).

 

Applications:
Immunohistochemistry:

Expression of  TRPC3 in rat DRG

Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPC3-ATTO-594 antibody (#ACC-016-AR), (1:50). Staining is present in neuronal cell bodies. Hoechst 33342 is used as the counterstain.


References:
1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362
2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
3. Clapham, D.E. (2003) Nature 426, 517.
4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707.
5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
6. Vazquez, G. et al. (2003) J. Biol.Chem. 278, 21649.
7. Venkatachalam, K. et al. (2003) J. Biol.Chem. 278, 29031.
For research purposes only, not for human use.