Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Netrin-3 Blocking Peptide (#BLP-NR123) is the original antigen used for immunization during Anti-Netrin-3 Antibody (#ANR-123) generation. The blocking peptide binds and ‘blocks’ Anti-Netrin-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-Netrin-3 Antibody (#ANR-123), (1:400).
3-4. Anti-Netrin-3 Antibody, preincubated with Netrin-3 Blocking Peptide (BLP-NR123).
Western blot analysis of human U-87 MG glioma cell line lysates (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):1-2. Anti-Netrin-3 Antibody (#ANR-123), (1:200).
3-4. Anti-Netrin-3 Antibody, preincubated with Netrin-3 Blocking Peptide (BLP-NR123).
Expression of Netrin-3 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Netrin-3 Antibody (#ANR-123), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn region showed Netrin-3 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with Netrin-3 Blocking Peptide (BLP-NR123), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CRPARGSYRISLKK, corresponding to amino acid residues 445 - 458 of mouse Netrin-3 (Accession Q9R1A3).
Accession (Uniprot) Number Q9R1A3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
