Overview
Type: Synthetic peptide
Form: Lyophilized Powder
CART Blocking Peptide (#BLP-NT047) is the original antigen used for immunization during Anti-CART Antibody (#ANT-047) generation. The blocking peptide binds and ‘blocks’ Anti-CART primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-CART Antibody (#ANT-047), (1:200).
3-4. Anti-CART Antibody, preincubated with CART Blocking Peptide (BLP-NT047).
Expression of CART in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-CART Antibody (#ANT-047), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CART immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with CART Blocking Peptide (BLP-NT047), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of CART in mouse parietal cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-CART Antibody (#ANT-047), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CART immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with CART Blocking Peptide (BLP-NT047), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CDAGEQ(S)AVRKGAR, corresponding to amino acid residues 95 - 108 of mouse CARTPT (Accession P56388).
Accession (Uniprot) Number P56388
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
