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Na+/H+ Exchanger Regulatory Factor-1 (NHERF-1): A PDZ-Domain Containing Protein Adaptor

Introduction

The Na+/H+ exchanger regulatory factor (NHERF-1 or EBP-50) is a 55 kD cytoplasmic protein adaptor that recruits a wide variety of cellular proteins. Many of the interacting proteins do so through the two tandem PDZ domains (protein-binding domains conserved in the mammalian synaptic protein PSD-95/DlgA/ZO-1) and the C-terminal ERM (ezrin, radixin, moesin) binding region.

NHERF-1 was first identified as an adaptor necessary for the function of the Na+/H+ exchanger isoform 3(NHE3) in renal apical cells1. Since then it has been identified in cells of epithelial origin in several tissues such as gastrointestinal and lung. NHERF-1 has been shown to interact with a growing number of proteins including ion channels (ROMK, CFTR, TRPC4 and TRPC6), G-protein coupled receptors, (P2Y1 ) growth factor receptors (PDGFR), phospholipase C isoforms (PLCβ1, PLCβ2, PLCβ3), non-receptor protein tyrosine kinases (YAP65) and several cytoskeletal proteins that link membrane proteins to the underlying actin cytoskeleton2. Recently it has been showed that NHERF-1 expression was elevated in breast tumors, when compared to the expression in adjacent normal tissue3.

New Antibody to NHERF-1

Our Anti-NHERF-1/EBP50 Antibody (#APZ-006) was developed against an epitope corresponding to residues 260-274 of rat NHERF-1 (Accession number Q9JJ19).

The epitope is located between the second PDZ domain and the C-terminal ERM binding region. The chosen rat epitope is highly homologous to the mouse (14 out of 15 residues) and human (13 out of 15 residues) NHERF-1 counterpart. The antibody is specific for NHERF-1 with no cross reactivity with the related protein NHERF-2.

new
Western blotting of rat kidney membranes:
1. Anti- NHERF-1/EBP50 Antibody (#APZ-006) (1:200).2. Anti- NHERF-1/EBP50 Antibody, preincubated with the control peptide antigen.

References

  1. Reczek, D. et al. (1997) J. Cell Biol. 139, 169.
  2. Voltz, J.W. et al. (2001) Oncogene 20, 6309.
  3. Stemmer-Rachamimov, A.O. et al. (2001) Am. J. Pathol. 158, 57.