Extracellular antibodies are ideal research tools for cell-surface proteins.
No permeabilization/fixation because using extracellular antibodies allows you to work with live cells!
Benefits of live cell imaging:
✓ Monitor cell movement
✓ Study protein transport across the cell
✓ Investigate protein internalization
ASIC channels belong to the ENaC/Degenerin superfamily and are proton-gated ion channels which are not voltage-dependent. ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3, ASIC4, and ASIC5.
ENaC channels are composed of three structurally related subunits: α, β, and γ which mostly coassemble as heteromeric structures in order to form functional channels. These channels are highly sensitive to amiloride and are hence commonly termed amiloride-sensitive channels.