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Multiplex Staining Products

Alomone Labs has developed two innovative platforms for studying protein-protein interaction and/or protein co-localization in complex immunostaining or immunoprecipitation protocols:

 

ATTO-Conjugated Antibodies: These antibodies can be used in immunohistochemistry (IHC) and immunocytochemistry (ICC) with same species antibodies.

 

Save time and resources with ONE antibody.

 

Benefits of immunostaining with ATTO-conjugated antibodies:
Direct immuno-fluorescence
Secondary antibodies are not necessary
Ideal for immuno-colocalization with same species/host antibodies

 

More on ATTO dyes

 

Guinea Pig-Raised Antibodies: Antibodies raised in guinea pig can be used with any non-guinea pig second species antibodies (e.g. antibodies raised in mouse, rabbit, goat, etc.) in complex immunostaining or immunoprecipitation protocols. Our line of guinea pig raised antibodies can be combined with any of our rabbit-raised or ATTO-conjugated antibodies in complex immunostaining protocols.

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Sphingosine 1-Phosphate Receptors

ATTO-Conjugated Antibodies

Sphingosine 1-phosphate (S1P) is an active byproduct of sphingomyelin metabolism. Upon secretion, it circulates in the blood via its binding to high-density lipoproteins and albumin. When S1P reaches its target cell it could activate 5 different high affinity receptors belonging to the G-protein coupled receptor superfamily: Sphingosine 1-phosphate receptors, termed S1PR1-5. Stimulation of S1PR receptors triggers a cascade of signaling events depending on the receptor and on the G-protein it couples. Through the different signaling pathways these receptors activate, S1PR receptors are implicated in adherens junction assembly, cytoskeletal changes, cell migration, proliferation and apoptosis.

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