Extracellular antibodies are ideal research tools for cell-surface proteins.
No permeabilization/fixation because using extracellular antibodies allows you to work with live cells!
Benefits of live cell imaging:
✓ Monitor cell movement
✓ Study protein transport across the cell
✓ Investigate protein internalization
Maintaining physiological intracellular pH values is vital for most cells. The Na+/H+ exchanger family (NHE and also known as SLC9 family) is widely expressed and has a key role in protecting cells against acidification. There are currently six NHE transporters identified in mammalian cells.