Corticotropin-Releasing Factor Receptors

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

9 Results
  • RAMP2 (extracellular) Blocking Peptide
    RAMP2 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-RAMP2 (extracellular) Antibody
    • Product Code: BLP-RR022
    • Short Description: A Blocking Peptide for Anti-RAMP2 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: RAMP2
  • RAMP1 (extracellular) Blocking Peptide
    RAMP1 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-RAMP1 (extracellular) Antibody
    • Product Code: BLP-RR021
    • Short Description: A Blocking Peptide for Anti-RAMP1 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: RAMP1
  • CRF2/CRHR2 (extracellular) Blocking Peptide
    CRF2/CRHR2 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-CRF2/CRHR2 (extracellular) Antibody
    • Product Code: BLP-CR052
    • Short Description: A Blocking Peptide for Anti-CRF2/CRHR2 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: CRHR2
  • CRF1/CRHR1 (extracellular) Blocking Peptide
    CRF1/CRHR1 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-CRF1/CRHR1 (extracellular) Antibody
    • Product Code: BLP-CR050
    • Short Description: A Blocking Peptide for Anti-CRF1/CRHR1 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: CRHR1
  • Anti-RAMP2 (extracellular) Antibody
    Anti-RAMP2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP2
    • Product Code: ARR-022
    • Short Description: A Rabbit Polyclonal Antibody to RAMP2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2
  • Anti-RAMP1 (extracellular) Antibody
    KO Validate'
    Anti-RAMP1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP1
    • Product Code: ARR-021
    • Short Description: A Rabbit Polyclonal Antibody to RAMP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Receptor activity-modifying protein 1, Calcitonin receptor-like receptor activity-modifying protein 1
  • Anti-CRF2/CRHR2 (extracellular) Antibody
    Anti-CRF2/CRHR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 2
    • Product Code: ACR-052
    • Short Description: A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Corticotropin-releasing factor receptor 2, CRF-R-2, CRF2 receptor beta isoform, Corticotropin-releasing hormone receptor 2, CRH receptor 2 variant B
  • Anti-CRF1/CRHR1 (extracellular)-FITC Antibody
    Anti-CRF1/CRHR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACR-050-F
    • Short Description: A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1
  • Anti-CRF1/CRHR1 (extracellular) Antibody
    Anti-CRF1/CRHR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 1
    • Product Code: ACR-050
    • Short Description: A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1
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