ATP-Binding Cassette Transporter Family

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

4 Results
  • ABCB4/MDR3 Blocking Peptide
    ABCB4/MDR3 Blocking Peptide
    A Blocking Peptide for Anti-ABCB4/MDR3 Antibody
    • Product Code: BLP-MT023
    • Short Description: A Blocking Peptide for Anti-ABCB4/MDR3 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: ABCB4
  • Anti-ABCB4/MDR3 Antibody
    Anti-ABCB4/MDR3 Antibody
    A Rabbit Polyclonal Antibody to ABCB4/MDR3
    • Product Code: AMT-023
    • Short Description: A Rabbit Polyclonal Antibody to ABCB4/MDR3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: ATP binding cassette subfamily B member 4, Multidrug resistance protein 3, P-glycoprotein 2, Pgy2
  • Anti-Human ABCB1/MDR1 Antibody
    Anti-Human ABCB1/MDR1 Antibody
    A Rabbit Polyclonal Antibody to Multidrug Resistance Protein 1
    • Product Code: AMT-021
    • Short Description: A Rabbit Polyclonal Antibody to Multidrug Resistance Protein 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Western blot
    • Target: Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170
  • Human ABCB1/MDR1 Blocking Peptide
    Human ABCB1/MDR1 Blocking Peptide
    A Blocking Peptide for Anti-Human ABCB1/MDR1 Antibody
    • Product Code: BLP-MT021
    • Short Description: A Blocking Peptide for Anti-Human ABCB1/MDR1 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: ABCB1
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