Ca+2 ATPases

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

20 Results
  • Anti-PMCA1 Antibody
    Anti-PMCA1 Antibody
    A Rabbit Polyclonal Antibody to PMCA1 ATPase
    • Product Code: ACP-005
    • Short Description: A Rabbit Polyclonal Antibody to PMCA1 ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Plasma membrane Ca2+ ATPase 1, ATP2B1
  • Anti-PMCA2 Antibody
    Anti-PMCA2 Antibody
    A Rabbit Polyclonal Antibody to PMCA2 ATPase
    • Product Code: ACP-002
    • Short Description: A Rabbit Polyclonal Antibody to PMCA2 ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Plasma membrane Ca2+ ATPase 2, ATP2B2
  • Anti-PMCA3 Antibody
    Anti-PMCA3 Antibody
    A Rabbit Polyclonal Antibody to PMCA3 ATPase
    • Product Code: ACP-003
    • Short Description: A Rabbit Polyclonal Antibody to PMCA3 ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Plasma membrane Ca2+ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1
  • Anti-PMCA4 Antibody
    Anti-PMCA4 Antibody
    A Rabbit Polyclonal Antibody to PMCA4 ATPase
    • Product Code: ACP-004
    • Short Description: A Rabbit Polyclonal Antibody to PMCA4 ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Plasma membrane Ca2+ ATPase 4, Matrix-Remodeling-Associated Protein 1, ATP2B4
  • Anti-SERCA1 Antibody
    Anti-SERCA1 Antibody
    A Rabbit Polyclonal Antibody to SERCA1 Pump
    • Product Code: ACP-011
    • Short Description: A Rabbit Polyclonal Antibody to SERCA1 Pump
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, Calcium pump 1, ATP2A1
  • Anti-SERCA2 Antibody
    Anti-SERCA2 Antibody
    A Rabbit Polyclonal Antibody to SERCA2 Pump
    • Product Code: ACP-012
    • Short Description: A Rabbit Polyclonal Antibody to SERCA2 Pump
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2
  • Anti-SERCA3 Antibody
    Anti-SERCA3 Antibody
    A Rabbit Polyclonal Antibody to SERCA3 Pump
    • Product Code: ACP-014
    • Short Description: A Rabbit Polyclonal Antibody to SERCA3 Pump
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, Calcium pump 3, ATP2A3
  • Chelerythrine chloride
    Bioassay Tested
    Chelerythrine chloride
    An Inhibitor of Protein Kinase C, SERCA Pump, and P2X7 Receptor
    • Product Code: C-400
    • Short Description: An Inhibitor of Protein Kinase C, SERCA Pump, and P2X7 Receptor
    • CAS No.: 3895-92-9
    • MW: 383.84
  • NS-1619
    Bioassay Tested
    NS-1619
    Selective and Potent Enhancer of KCa1.1 Channels and an Inhibitor of SERCA Pumps
    • Product Code: N-105
    • Short Description: Selective and Potent Enhancer of KCa1.1 Channels and an Inhibitor of SERCA Pumps
    • CAS No.: 153587-01-0
    • MW: 362.2
  • Ochratoxin A
    Ochratoxin A
    A Potent Modulator of Ca2+ ATPase Pump
    • Product Code: O-400
    • Short Description: A Potent Modulator of Ca2+ ATPase Pump
    • CAS No.: 303-47-9
    • MW: 403.8
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