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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

20 Results
  • Anti-OCTN2/SLC22A5 Antibody
    Anti-OCTN2/SLC22A5 Antibody
    A rabbit polyclonal antibody to OCTN2/SLC22A5
    • Product Code: ACT-015
    • Short Description: A rabbit polyclonal antibody to OCTN2/SLC22A5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SLC22A5
  • Na<sub>V</sub>1.2 XenoBlot&trade; Positive Control
    NaV1.2 XenoBlot™ Positive Control
    SCN2A (human NaV1.2) protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Product Code: XB-015
    • Short Description: SCN2A (human NaV1.2) protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Applications: Western blot
  • Ca<sub>V</sub>3.1 XenoBlot&trade; Positive Control
    CaV3.1 XenoBlot™ Positive Control
    CACNA1G (rat CaV3.1) protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Product Code: XB-014
    • Short Description: CACNA1G (rat CaV3.1) protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Applications: Western blot
  • Anti-EphA5 (extracellular) Antibody
    Anti-EphA5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA5 (Extracellular)
    • Product Code: AER-015
    • Short Description: A Rabbit Polyclonal Antibody to EphA5 (Extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: EPHA5
  • Anti-C3aR1 (extracellular)-mFluor™ Violet 450 Antibody
    Anti-C3aR1 (extracellular)-mFluor™ Violet 450 Antibody
    A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450 fluorophore.
    • Product Code: AAR-036-V
    • Short Description: A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450 fluorophore.
    • Host: Rabbit
    • Label: mFluor™ Violet 450
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Na<sub>V</sub>1.5 XenoBlot&trade; Positive Control
    NaV1.5 XenoBlot™ Positive Control
    SCN5A (human NaV1.5) protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Product Code: XB-005
    • Short Description: SCN5A (human NaV1.5) protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Applications: Western blot
  • Maurocalcine
    Bioassay Tested
    Maurocalcine
    A cell-permeant, potent and selective agonist for the RyR1 ryanodine receptor, inducing massive intracellular calcium release.
    • Product Code: STM-105
    • Short Description: A cell-permeant, potent and selective agonist for the RyR1 ryanodine receptor, inducing massive intracellular calcium release.
    • Applications: Muscle Physiology Assays, Electrophysiology, Calcium imaging assay
    • CAS No.: 269745-22-4
    • MW: 3858.6 Da
  • Anti-OXGR1/GPR99 (extracellular) Antibody
    Anti-OXGR1/GPR99 (extracellular) Antibody
    A rabbit polyclonal antibody to OXGR1/GPR99 (extracellular)
    • Product Code: AOR-017
    • Short Description: A rabbit polyclonal antibody to OXGR1/GPR99 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, AKGR, GPR80, P2RY15, P2Y15, CysLTR3
  • TRPC6 XenoBlot&trade; Positive Control
    TRPC6 XenoBlot™ Positive Control
    TRPC6 protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Product Code: XB-012
    • Short Description: TRPC6 protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Applications: Western blot
  • Na<sub>V</sub>1.8 XenoBlot&trade; Positive Control
    NaV1.8 XenoBlot™ Positive Control
    SCN10A (human NaV1.8) protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Product Code: XB-016
    • Short Description: SCN10A (human NaV1.8) protein, overexpressed in Xenopus oocytes, used as a positive control for Western blot analysis.
    • Applications: Western blot
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