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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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41 Results
  • Hm1a-ATTO Fluor 647N
    Bioassay Tested
    Hm1a-ATTO Fluor 647N
    A fluorescent probe for imaging and sensitive detection of NaV1.1 voltage-gated sodium channels
    • Product Code: STH-600-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of NaV1.1 voltage-gated sodium channels
    • Label: ATTO 647N
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 4625 Da
  • ATX-II-ATTO Fluor-647N
    Bioassay Tested
    ATX-II-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of NaV sodium channels
    • Product Code: STA-700-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of NaV sodium channels
    • Label: ATTO 647N
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 5561 Da
  • µ/ω-TRTX-Tap1a
    Bioassay Tested
    µ/ω-TRTX-Tap1a
    A potent blocker/gating-modifier of multiple NaV and T-type (CaV3.1, CaV3.2) channels
    • Product Code: STT-600
    • Short Description: A potent blocker/gating-modifier of multiple NaV and T-type (CaV3.1, CaV3.2) channels
    • Applications: Electrophysiology
    • MW: 4182.7 Da
  • GTx1-15
    Bioassay Tested
    GTx1-15
    A blocker of voltage-gated Na+ and T-Type Ca2+ channels
    • Product Code: STT-300
    • Short Description: A blocker of voltage-gated Na+ and T-Type Ca2+ channels
    • Applications: Electrophysiology
    • MW: 4073.8 Da
  • Phlotoxin 1
    Bioassay Tested
    Phlotoxin 1
    A potent blocker of NaV1.7 channels and inhibitor of other NaV subtypes
    • Product Code: STP-800
    • Short Description: A potent blocker of NaV1.7 channels and inhibitor of other NaV subtypes
    • Applications: Electrophysiology
    • MW: 4057.7 Da
  • Phrixotoxin-3
    Bioassay Tested
    Phrixotoxin-3
    A potent blocker of NaV1.2 and NaV1.6 channels
    • Product Code: STP-720
    • Short Description: A potent blocker of NaV1.2 and NaV1.6 channels
    • Applications: Electrophysiology
    • CAS No.: 880886-00-0
    • MW: 4058.8 Da
  • ProTx-III
    Bioassay Tested
    ProTx-III
    A potent blocker/gating modifier of NaV1.7 channels
    • Product Code: STP-150
    • Short Description: A potent blocker/gating modifier of NaV1.7 channels
    • Applications: Electrophysiology
    • MW: 3802.5 Da
  • Jingzhaotoxin-II
    Bioassay Tested
    Jingzhaotoxin-II
    A potent blocker/gating modifier of cardiac NaV1.5 channels
    • Product Code: STJ-150
    • Short Description: A potent blocker/gating modifier of cardiac NaV1.5 channels
    • Applications: Electrophysiology
    • MW: 3561 Da
  • Hm1b Toxin
    Bioassay Tested
    Hm1b Toxin
    A potent activator of neuronal NaV1.1 and NV1.3 channels
    • Product Code: STH-655
    • Short Description: A potent activator of neuronal NaV1.1 and NV1.3 channels
    • Applications: Electrophysiology
    • MW: 3895.4 Da
  • Hm1a Toxin
    Bioassay Tested
    Hm1a Toxin
    A potent and selective activator of NaV1.1 channels
    • Product Code: STH-601
    • Short Description: A potent and selective activator of NaV1.1 channels
    • Applications: Electrophysiology
    • MW: 3996.4 Da
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